Anti-SATB2 antibody produced in rabbit
SIGMA/HPA001042 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: SATB2 Antibody - Anti-SATB2 antibody produced in rabbit; Satb2 Antibody; Anti-APXL3; Anti-FLJ21474; Anti-KIAA1034; Anti-KIAA1481; Anti-SATB homeobox 2; Anti-SHRM; Anti-ShrmL
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human cerebral cortex, colon, liver and rectum using Anti-SATB2 antibody HPA001042 (A) shows similar protein distribution across tissues to independent antibody HPA029543 (B).
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human colon and liver tissues using HPA001042 antibody. Corresponding SATB2 RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunohistochemical staining of human colorectal cancer shows strong nuclear positivity in tumor cells.
Immunohistochemistry Immunohistochemical staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.
Western Blotting Western blot analysis in human cell line HEL.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | VSQAVFARVAFNRTQGLLSEILRKE |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunohistochemistry: 1:1000-1:2500 | |
| UniProt accession no. | Q9UPW6  |
| Application: | Anti-SATB2 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Biochem/physiol Actions: | Special AT-rich sequence-binding protein 2 (SATB2) binds to nuclear matrix-attachment regions (MAR), displays functional versatility in central nervous development, especially corpus callosum and pons formation, cancer development and prognosis, as well as in immune regulation. The gene is a nuclear matrix-associated transcription factor and epigenetic regulator that is involved in osteoblastic differentiation. It is expressed in the glandular epithelial cells of the lower gastrointestinal tract. It plays a critical role in osteoblast lineage commitment and might be involved in the development and progression of laryngeal squamous cell carcinoma (LSCC) as a tumor suppressor. SATB2 is a marker of osteoblastic differentiation in benign and malignant mesenchymal tumours and colorectal adenocarcinomas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | SATB2 (SATB homeobox 2) is an AT rich DNA-binding protein. It is a homologue of SATB1 protein. This gene is localized to human chromosome 2q33.1, and is highly conserved across different species. |
| Immunogen: | SATB homeobox 2 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST76122 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |