Anti-GOLGA5 antibody produced in rabbit
SIGMA/HPA000992 - affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-Golgin subfamily A member 5 antibody produced in rabbit; Anti-Golgin-84 antibody produced in rabbit; Anti-RET-fused gene 5 protein antibody produced in rabbit; Anti-Ret-II protein antibody produced in rabbit
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human epididymis and skeletal muscle tissues using HPA000992 antibody. Corresponding GOLGA5 RNA-seq data are presented for the same tissues.
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human cerebellum, epididymis, stomach and testis using Anti-GOLGA5 antibody HPA000992 (A) shows similar protein distribution across tissues to independent antibody HPA063876 (B).
Enhanced Validation-RNAi Knockdown Western blot analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe nos. 1 and 2, using Anti-GOLGA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry Immunofluorescence staining of mouse mesencephalic reticular formation shows strong immunoreactivity in a subset of neurons.
Immunohistochemistry Immunofluorescence staining of mouse hippocampus shows immunoreactivity in a subset of neurons in the CA3 area.
Immunohistochemistry Immunofluorescence staining of mouse thalamus shows positivity in neuronal cell bodies in ventral anterior nucleus.
Immunohistochemistry Immunofluorescence staining of mouse motor cortex shows strong cytoplasmic immunoreactivity in neurons.
Immunohistochemistry Immunofluorescence staining of mouse globus pallidus shows positivity in neuronal cell bodies.
Immunohistochemistry Immunohistochemical staining of human epididymis shows strong positivity in Golgi apparatus in glandular cells.
Immunohistochemistry Immunohistochemical staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry Immunohistochemical staining of human stomach shows moderate to strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry Immunohistochemical staining of human cerebellum shows moderate granular cytoplasmic positivity in Purkinje cells.
Immunofluorescence Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent RNAi knockdown independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | SSVNPSVTTIKTIEENSFGSQTHEA |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:200-1:500 | |
| UniProt accession no. | Q8TBA6  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunocytochemistry (1 paper) |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| Immunogen: | Golgin subfamily A member 5 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST70366 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |