Anti-GLA antibody produced in rabbit
SIGMA/HPA000966 - Ab2, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-α-D-Galactosidase A antibody produced in rabbit; Anti-α-D-Galactoside galactohydrolase antibody produced in rabbit; Anti-α-Galactosidase A precursor antibody produced in rabbit; Anti-Agalsidase alfa antibody produced in rabbit; Anti-Melibiase antibody produced in rabbit
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human adrenal gland, colon, liver and testis using Anti-GLA antibody HPA000966 (A) shows similar protein distribution across tissues to independent antibody HPA000237 (B).
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human seminal vesicle and pancreas tissues using Anti-GLA antibody. Corresponding GLA RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunohistochemical staining of human seminal vesicle shows high expression.
Immunohistochemistry Immunohistochemical staining of human pancreas shows low expression as expected.
Immunohistochemistry Immunohistochemical staining of human liver using Anti-GLA antibody HPA000966.
Immunohistochemistry Immunohistochemical staining of human colon using Anti-GLA antibody HPA000966.
Immunohistochemistry Immunohistochemical staining of human adrenal gland using Anti-GLA antibody HPA000966.
Immunohistochemistry Immunohistochemical staining of human testis using Anti-GLA antibody HPA000966.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | FDGCYCDSLENLADGYKHMSLALNR |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:200- 1:500 |
| UniProt accession no. | P06280  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | α-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal α-galactosyl moieties from glycolipids and glycoproteins, encoded by the GLA gene. Deficit of α-galactosidase A enzyme causes Fabry disease, an X-linked inherited metabolic condition (leads to glycosphingolipid storage, mainly globotriaosylceramide). Missense mutations in the α-galactosidase A (GLA) gene comprises the majority of mutations that are responsible for Fabry disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | GLA (galactosidase α) gene is localized to human chromosome Xq22.1, which codes for a precursor protein of 429 amino acids. This protein is then processed and glycosylated to form a mature protein of 398 amino acids. This protein is localized to lysosomes and exists as a homodimer. |
| Immunogen: | α-Galactosidase A precursor recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST74068 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |