Histopaque®-1077 Hybri-Max™
SIGMA/H8889 - liquid, sterile-filtered, BioReagent, suitable for hybridoma
Synonym: Histopaque 1077
Product Type: Chemical
| density | 1.077 at 25 °C (relative density)(lit.) |
| form | liquid |
| impurities | endotoxin, tested |
| product line | BioReagent |
| Quality Level | 400  |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | hybridoma: suitable |
| single cell analysis: suitable |
| Application: | Histopaque®-1077 Hybri-Max™ has been used to isolate: • peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation • immune cells (lymphocytes) from peripheral blood • PBMCs from blood |
| General description: | Histopaque®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm3. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells. |
| Legal Information: | Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Hybri-Max is a trademark of Sigma-Aldrich Co. LLC |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 - H350 |
| Precautionary statements | P202 - P261 - P272 - P280 - P302 + P352 - P308 + P313 |
| Hazard Codes | Xi |
| Risk Statements | 36/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.077 at 25 °C (relative density)(lit.) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352201 |