Interleukin-2 human
SIGMA/H7041 - IL-2, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin tested
Synonym: Interleukin-2; T-cell growth factor
MDL Number: MFCD00165721
Product Type: Chemical
Bioassay EC50 is defined as the effective concentration of Interleukin-2 Human that elicits a 50% attachment factor in cell based bioassay.
| biological source | human |
| form | lyophilized powder |
| mol wt | monomer 15 kDa (glycosylated) |
| packaging | pkg of 10 μg |
| pkg of 100 μg | |
| pkg of 5X10 μg | |
| potency | ≤5.0 ng/mL ED50 |
| quality | endotoxin tested |
| Quality Level | 100  |
| recombinant | expressed in HEK 293 cells |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P60568  |
| Analysis Note: | The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells (mouse cytotoxic T cell line). |
| Application: | Interleukin-2 human has been used to culture peripheral blood mononuclear cells (PBMC). It has been used to activate effector cell (PBMC) and to study antibody-dependent cell-mediated cytotoxicity. |
| Biochem/physiol Actions: | Interleukin-2 (IL-2) is an immunomodulatory factor produced by certain subsets of T lymphocytes. This lymphokine promotes long term growth of activated T cells and related cell types. IL-2 plays a role in the activation and proliferation of NK cells, induces γ-interferon and B cell growth factor secretion, and modulates the expression of the IL-2 receptor. |
| Biochem/physiol Actions: | Interleukin-2 (IL-2) is essential for proliferation of natural killer (NK) cells, lymphocytes and macrophages. It plays a key role in the immune system functions such as NK cell-stimulated immune responses. IL-2 also mediates the formation and essential functions of regulatory T cells. It is implicated in autoimmune disease. IL-2 is associated with apoptosis and thus, possesses antitumor response. IL-2 mediates CD41+ T-cells differentiation into T-helper cell 1 and 2 effector subsets, preventing T-helper 17 differentiation. |
| General description: | Interleukin-2 (IL-2) is an α-helical cytokine belonging to T-helper cell type 1. It has a molecular weight of 15kDa. The IL2 gene is mapped to human chromosome 4q27. IL-2 is originally called as T cell growth factor. |
| Packaging: | 10 μg in poly bottle |
| Preparation Note: | Human Interleukin-2 (IL-2) expressed in human HEK 293 cells is a glycosylated monomer with a molecular mass of 15 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |