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HIV Protease Substrate 1

SIGMA/H6660

Synonym: Arg-Glu(EDANS)-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys(DABCYL)-Arg

MDL Number: MFCD00283442
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-H6660-1MG 1 mg
$658.00
1/EA
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assay ≥95% (HPLC)
form powder
mol wt 2016
Quality Level 200 
solubility DMSO: soluble
storage temp. −20°C
Application: HIV Protease Substrate 1 has been used:
• to study protein structure-function relationships and in the fluorescent assay
• to evaluate protease inhibitors and for in vitro detection of the HIV-1 protease activity
• and in protease kinetics to study its enzymatic degradation rates

Biochem/physiol Actions: HIV Protease Substrate 1 is a synthetic peptide sequence that contains the cleavage site (Tyr-Pro) for the human immunodeficiency virus (HIV) Protease. It has two covalently modified amino acids for the detection of cleavage. One modification is the addition of the fluorophore 5-(2-aminoethylamino)-1-naphthalene sulfonate (EDANS) to the glutamic acid residue, while the other one includes the addition of the acceptor chromophore 4c-dimethylaminoazobenzene-4-carboxylate (DABCYL) to the lysine residue. The modified amino acids are on opposite sides of the cleavage site. Spatial orientation and overlap of the DABCYL absorbance with the EDANS emission permit resonance energy transfer between the two moieties. However, when the peptide is cleaved by the HIV protease, the DABCYL group is no longer proximal to the fluorophore.
Biochem/physiol Actions: Substrate for endopeptidases
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥95% (HPLC)
Storage Temp. −20°C
UNSPSC 12352204

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