Anti-HA−Peroxidase antibody, Mouse monoclonal
SIGMA/H6533 - clone HA-7, purified from hybridoma cell culture
Synonym: Monoclonal Anti-HA; Anti-HA; Anti-Influenza Hemagglutinin
MDL Number: MFCD00803873
Product Type: Chemical
Western Blotting HA tagged fusion protein were separated on SDS-PAGE. The antibody was probed and developed with Monoclonal Anti-HA-Peroxidase antibody produced in Mouse, Clone: HA-7 (Cat. No. H6533). Lanes 1. 1:4,000 antibody 2. 1:8,000 antibody 3. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HA-7, monoclonal |
| concentration | 5-11 mg/mL |
| conjugate | peroxidase conjugate |
| form | lyophilized powder |
| packaging | vial of 0.5 mL |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| technique(s) | western blot: 1:4,000 using using HA tagged fusion protein expressed in bacteria, and ECL immunoblotting detection reagent |
| Application: | Monoclonal Anti-HA-Peroxidase antibody produced in mouse has been used: • in immunopreciptation and immunostaining of human member 9 of solute carrier family 38 SLC38A9 transfected in HeLa cells • in immunoblot detection in transfected A375 cells • is suitable for immunoblotting of nucleosome assembly protein NAP1L1 |
| Biochem/physiol Actions: | The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells. |
| General description: | Influenza A virus, a RNA virus, encodes two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA). HA stalk domain based epitope region antibody is effective in neutralizing divergent influenza virus strains. |
| General description: | The mouse monoclonal Anti-HA-Peroxidase antibody recognizes native and denatured-reduced forms of the HA-tagged proteins. It also reacts with the N- or C-terminal HA-tagged fusion proteins expressed in E. coli and mammalian cells. |
| Immunogen: | synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH |
| Physical form: | Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT. |
| Preparation Note: | Reconstitute in .5mL of distilled water. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |