Monoclonal Anti-Haptoglobin antibody produced in mouse
SIGMA/H6395 - clone HG-36, ascites fluid
MDL Number: MFCD00162410
Product Type: Chemical
ELISA Monoclonal Anti-Haptoglobin antibody produced in Mouse, Clone: HG-36 (Cat. No. H6395), followed by 1:1,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect Haptoglobin by indirect ELISA.Reactivity is observed with Human Haptoglobin but not with α-1-acid glycoprotein and Human IgG
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HG-36, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:4,000 |
| UniProt accession no. | P00738  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Monoclonal Anti-Haptoglobin antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA) to determine plasma haptoglobin (Hp) levels and to probe duplicate dot blot strips for dot blot assay. It has been used in affinity purification of human high-density lipoprotein (HDL) to obtain trypanosome lytic factor (TLF) and also to prepare an affinity resin. |
| Biochem/physiol Actions: | The antibody is specific for the 3 major haplotypes of human haptoglobin (Types 1-1, 2-1 and 2-2). It does not cross-react with human IgG, transferrin, C-reactive protein, α1-acid glycoprotein, albumin or hemoglobin using ELISA or immunoblotting methods. The antibody recognizes human haptoglobin as purified antigen, in human serum, or when complexed with human hemoglobin in a native or denatured, but non-reduced state. |
| Biochem/physiol Actions: | The most characteristic property of haptoglobin is its ability to form stable complexes with extra-corpuscular-free native hemoglobin that has been released during hemolysis and is thus thought to prevent iron loss through urinary excretion and to also prevent renal damage from the hemoglobin. In addition, the native form or normal plasma haptoglobin has been shown to exhibit a broad spectrum of immunosuppressive effects in the immune system. Measurement of haptoglobin levels may therefore be used to monitor the progress of inflammatory reactions and assess the efficiency of test drugs. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal anti-Human Haptoglobin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Haptoglobin (Hp) is a serum α 2-glycoprotein that exists as a tetramer, composed of two smaller identical α chains and two larger identical β chains. The α-chains are linked to each other by a disulfide bond and each β-chain is similarly linked to an α-chain. Plasma haptoglobin is structurally similar to serum immunoglobulins. Haptoglobin is found in normal plasma at a concentration of 0.3-1.9 mg/ml and accounts for 0.4-2.6% of the total plasma proteins. It is also one of the groups of plasma proteins known as acute phase reactants. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |