Hexokinase from Saccharomyces cerevisiae
SIGMA/H6380 - lyophilized powder, ≥350 units/mg protein, Protein ≥10 % by biuret
Synonym: ATP:D-
CAS Number: 9001-51-8
EC Number: 232-611-5
MDL Number: MFCD00131321
Product Type: Chemical
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| application(s) | life science and biopharma |
| biological source | Saccharomyces cerevisiae |
| color | white |
| composition | Protein, ≥10% biuret |
| foreign activity | |
| Creatine phosphokinase, myokinase, 6-phosphogluconic dehydrogenase and alcohol dehydrogenase ≤0.001% | |
| Glucose-6-phosphate dehydrogenase ≤0.005% | |
| Glutathione reductase ≤0.005% | |
| Phosphoglucose isomerase ≤0.002% | |
| form | lyophilized powder |
| manufacturer/tradename | Sigma-Aldrich |
| mol wt | dimer 110 kDa |
| pH | 6 |
| Quality Level | 200  |
| specific activity | ≥350 units/mg protein |
| storage temp. | 2-8°C |
| technique(s) | activity assay: suitable |
| UniProt accession no. | P04806  |
| P04807 |
| Application: | Hexokinase from Saccharomyces cerevisiae has been used: • to investigate its amyloid fibril forming tendency using biophysical methods • in tracking ATP depletion in peroxisomes and reticulocyte lysates in import assays • in the synthesis of Mg-ADP-actin from Mg-ATP actin monomers |
| Biochem/physiol Actions: | Catalyzes the phosphorylation of The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C). Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively. Molecular Weight: ~ 54 kDa (monomer) ~110 kDa (dimer) Optimal pH: 7.5 to 9.0 Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds. Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose |
| Biochem/physiol Actions: | Hexokinase II is a crucial enzyme in glucose metabolism. Deletion of gene in S. cerevisiae in the presence of high glucose displays oxidative growth and results in the production of high biomass as well as shortened diauxic shift. Hexokinase II is also involved in glucose repression. |
| General description: | Research area: Cell signalling. Hexokinase (HK) from yeast is a 486 amino-acid containing enzyme with a α/β fold. It comprises a large and small domain separated by an active site cleft. Conserved glycine residues are essential for ATP and glucose binding. The two isoforms are HKI and HKII encoded by HXK1 and HXK2 gene, respectively. |
| Other Notes: | One unit will phosphorylate 1.0 μmole of |
| Other Notes: | Predominantly the PII isoform, produced from an overexpressing yeast strain. |
| Packaging: | 1500 units in poly bottle |
| Physical form: | Lyophilized powder containing phosphate/citrate pH approx. 7.0 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥350 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 2.7.1.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |