Interleukin-1β human
SIGMA/H6291 - IL-1β, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture, endotoxin tested
Synonym: Interleukin-1β human; Lymphocyte activating factor; IL-1β; Lymphocyte activating factor
MDL Number: MFCD00131399
Product Type: Chemical
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| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | <1 EU/μg |
| mol wt | dimer 18-25 kDa (glycosylated) |
| packaging | pkg of 10 μg |
| potency | ≤0.05 ng/mL EC50 |
| quality | endotoxin tested |
| Quality Level | 100  |
| recombinant | expressed in HEK 293 cells |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01584  |
| Analysis Note: | The activity is determined by the dose-dependent stimulation of the proliferation of mouse D10S cells. |
| Biochem/physiol Actions: | Interleukin-1 (IL-1), originally known as Lymphocyte Activating Factor (LAF), activates T cell lymphocytes, which then proliferate and secrete interleukin-2 (IL-2). IL-1 is released primarily from stimulated macrophages and monocytes, but has also been shown to be released from several other types and is thought to play a key role in inflammatory and immune responses. Other synonyms for IL-1 include: endogenous pyrogen (EP), mitogenic protein (MP), Helper Peak-1 (HP-1), T Cell Replacing Factor III (TRF III or TRFM), B Cell Activating Factor (BAF) and B Cell Differentiation Factor (BDF) The two closely related agents Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) share 62% homology in amino acid sequence and elicit nearly identical biological responses. IL-1α and IL-1β are both approximately 17 kDa with some heterogeneity in the amount of glycosylation. |
| Biochem/physiol Actions: | Interleukin-1 activates T cell lymphocytes, which then proliferate and secrete interleukin-2 (IL-2). IL-1 is released primarily from stimulated macrophages and monocytes, but has also been shown to be released from several other types and is thought to play a key role in inflammatory and immune responses. |
| Legal Information: | HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc |
| Physical form: | Lyophilized from a 0.2 μm filtered solution of 1x PBS. |
| Preparation Note: | HumanKine Recombinant Human IL-1β, expressed in human HEK 293 cells, is a glycosylated monomer with a molecular mass of 18-25 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |