Hyaluronidase from sheep testes
SIGMA/H6254 - Type V, lyophilized powder, ≥1,500 units/mg solid
Synonym: Hyaluronate 4-glycanohydrolase; Hyaluronoglucosaminidase
CAS Number: 37326-33-3
EC Number: 253-464-3
MDL Number: MFCD00131351
Product Type: Chemical
Hyaluronidase specificity: Hyaluronic acid is a polymer of alternating residues of β-D-(1-3) glucuronic acid and β-D-(1-4)-N-acetylglucosamine.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: H6254-500MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: H6254-1G
| application(s) | diagnostic assay manufacturing |
| biological source | sheep testis |
| form | lyophilized powder |
| mol wt | 55 kDa |
| Quality Level | 200  |
| specific activity | ≥1,500 units/mg solid |
| storage temp. | −20°C |
| type | Type V |
| Application: | Hyaluronidase has been used: • as a component of enzyme mix for the isolation of sertoli and germ cells from seminiferous tubules • in the pre-treatment of deparaffinized adenomas sections for immunohistochemistry • in the digestion of umbilical cord blood vessel for the generation of umbilical cord stromal stem cell lines |
| Biochem/physiol Actions: | Hyaluronidase from sheep testis is commercially used to depolymerise hyaluronic acid and chondroitin-sulphate in connective tissue and cartilage respectively. |
| Biochem/physiol Actions: | These enzymes randomly cleave β-N-acetylhexosamine-(1−4 |
| General description: | Hyaluronidase is present in high amount in testis as it aids fertilization of the mammalian egg. Mammalian hyaluronidase has a catalytic epidermal growth factor (EGF)-like domain and a C-terminal cysteine rich region. |
| Other Notes: | One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes). |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 1 g in poly bottle |
| Packaging: | 500 mg in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1,500 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.35 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |