Anti-HSV antibody produced in rabbit
SIGMA/H6030 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Herpes Simplex Virus
MDL Number: MFCD02263028
Product Type: Chemical
Immunoblotting E. Coli extract expressing pet25B/lac/HSV/Hisx6 was separated on SDS-PAGE and probed with AIA To HSV (RB) (Cat. No. H6030). The antibody was developed with Protein A HRP (Cat. No. P8651) and ECL substrate. Antibody concentration 1.25 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 1.0 μg/mL |
| western blot: 2.5 μg/mL |
| Application: | Anti-HSV antibody was used to expand the repertoire of plasmids for PCR-mediated epitope tagging in yeast. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Chromatin immunoprecipitation (1 paper) Western Blotting (1 paper) |
| Biochem/physiol Actions: | Anti-HSV is developed in rabbits using a synthetic peptide (K-QPELAPEDPED) conjugated to KLH via the N-terminal lysine. The peptide corresponds to amino acids 290-300 of Glycoprotein D precursor which is an envelope component of herpes simplex virus. Anti-HSV antibody reacts specifically with HSV tagged fusion proteins, using immunoblotting and immunoprecipitation techniques. |
| General description: | Recognizes N- and C-terminal HSV fusion proteins. |
| Immunogen: | synthetic peptide corresponding to amino acids 290−300 of glycoprotein-D precursor, an envelop component of herpes simplex virus. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |