Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit
SIGMA/H5912 - IgG fraction of antiserum, buffered aqueous solution
Synonym: H2Ax Antibody; H2Ax Antibody - Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit; Anti-H2AXS139p
MDL Number: MFCD04974783
Product Type: Chemical
Immunoblotting
Whole cell extracts from staurosporine-treated NIH3T3 (Lane A) and HeLa (Lanes B, C, D) cells were separated on SDS-PAGE, blotted with Rabbit Anti-phospho-Histone H2AX [pSer139] (Cat. No. H5912) and developed using Goat Anti-Rabbit IgG Peroxidase (Cat. No. A0545) and chemiluminescent substrate. The antibody is specific for the pSer139 phosphorylated Histone H2AX as shown by the inhibition of antibody binding with the phosphorylated peptide and lack of inhibition with the non-phosphorylated peptide. (Lane A,C) Antibody dilution 1:1,000; (Lane C) Antibody dilution 1:1,000+ 5 μg/ml phospho-Histone H2AX [pSer139] peptide; (Lane D Antibody dilution 1:1,000+ 5 μg/ml non-phospho-Histone H2AX peptide.
Whole cell extracts from staurosporine-treated NIH3T3 (Lane A) and HeLa (Lanes B, C, D) cells were separated on SDS-PAGE, blotted with Rabbit Anti-phospho-Histone H2AX [pSer139] (Cat. No. H5912) and developed using Goat Anti-Rabbit IgG Peroxidase (Cat. No. A0545) and chemiluminescent substrate. The antibody is specific for the pSer139 phosphorylated Histone H2AX as shown by the inhibition of antibody binding with the phosphorylated peptide and lack of inhibition with the non-phosphorylated peptide. (Lane A,C) Antibody dilution 1:1,000; (Lane C) Antibody dilution 1:1,000+ 5 μg/ml phospho-Histone H2AX [pSer139] peptide; (Lane D Antibody dilution 1:1,000+ 5 μg/ml non-phospho-Histone H2AX peptide.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 15 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer139) |
| technique(s) | microarray: suitable |
| western blot: 1:1,000 using whole cell extracts of HeLa (human epitheloid carcinoma) cells or NIH3T3 mouse fibroblast cells treated with staurosporine | |
| UniProt accession no. | P16104  |
| Application: | Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit has been used in immunofluorescence and western blotting. |
| Biochem/physiol Actions: | H2AX is critical for facilitating the assembly of specific DNA-repair complexes on damaged DNA. Ataxia-telangiectasia-mut |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | H2AX comprises 2-25% of the total H2A complement in human cells, whereas in the budding yeast it constitutes virtually all of the H2A molecules. It has N-terminal and C-terminal region with a central globular domain. The C-terminal region has sites for modification including acetylation, biotinylation and phosphorylation. |
| Immunogen: | synthetic phosphorylated peptide corresponding to the C-terminus (amino acids 134-142) of human histone H2AX (pSer139). |
| Immunogen: | The synthetic peptide sequence is conjugated to KLH. The immunogen sequence is highly conserved (single amino acid substitution) in mouse histone H2AX. |
| Packaging: | 200 μL in glass insert |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |