Activin A human
SIGMA/H4666 - recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
Synonym: Activin A Protein; Growth Factor; Human Activin A
MDL Number: MFCD02685750
Product Type: Chemical
Activity as determined by the dose-dependent inhibition of proliferation of a mouse plasmocytoma-11 cell line
SDS_PAGE analysis using Coomassie blue staining
Activin A is processed In Vivo as a monomeric glycosylated protein. After proteolytic processing, the active form may exist as a disulfide-linked hetero- or homo dimer.
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | ≤1 EU/mg |
| mol wt | dimer 25 kDa (non-glycosylated) |
| packaging | pkg of 10 μg |
| pkg of 5x10 μg | |
| potency | ≤5 ng/mL EC50 |
| quality | endotoxin tested |
| Quality Level | 100  |
| recombinant | expressed in HEK 293 cells |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| Analysis Note: | The specific activity was determined by the dose-dependent inhibition of proliferation of the MPC-11 cell line (mouse plasmocytoma cell line). |
| Biochem/physiol Actions: | Activin-A participates in a diverse array of functions that include; hypothalamic/pituitary/go Activin-A stimulates glycogenolysis in isolated rat hepatocytes, Mine T ET. al. (1989) and elevates insulin release from rat pancreatic islets, Verspohl EJ et al. (1993). Activin-A stimulates insulin secretion in rat pancreatic islets, Totsuka Y, et al. (1988). Follistatin, an activin-binding protein, is a principle regulator of activin activity, de Winter JP, et al. (1996); however during embryogenesis, Cripto is an important noncompetitive activin antagonist that facilitates Nodal signaling, Kelber JA, et al. (2008). A role for activin-A as a regulator of cell proliferation was recognized by Gonzalez-Manchon C and Vale W. (1989); wherein Act-A inhibited the growth of and induced morphological changes in CHO-KI cells in culture, in a way similar to but less potent than TGF-β. Activin A is involved with the entire process of embryo development from germ cells thru embryonic development to adult tissues. It stimulates spermatogonial proliferation in germ-Sertoli cell cocultures, Mather JP, et al. (1990) and is a maturation factor for oocytes, Itoh M, et al. (1990). Activin A promotes proliferation of human luteinized preovulation granulose cells (ovarian granulose cells), Rabinovici J, et al. (1990). |
| Legal Information: | HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |