Anti-Histone Deacetylase 5 (HDAC5) antibody, Mouse monoclonal
SIGMA/H4538 - clone HDAC5-35, purified from hybridoma cell culture
Synonym: Anti-HD5
MDL Number: MFCD04040361
Product Type: Chemical
Immunoblot 3T3 cell extract (mouse fibroblasts) was separated by SDS-PAGE and probed with (Lane 1) or without (Lane 2) Monoclonal Anti-HDAC5 (clone HDAC5-35 Cat. No. H4538) followed by Goat Anti-Mouse, Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HDAC5-35, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~124 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 4-8 μg/mL using total cell extracts of NIH3T3 fibroblast cell or SW480 cell extract | |
| UniProt accession no. | Q9UQL6  |
| Application: | Anti-Histone Deacetylase 5 (HDAC5) antibody, Mouse monoclonal has been used in: • western blotting • immunostaining • enzyme-linked immunosorbent assay (ELISA) • immunocytochemistry • immunoprecipitation |
| Biochem/physiol Actions: | Histone deacetylase 5 (HDAC5) promotes progression of the cell cycle, cell proliferation and apoptosis. HDAC5 activity is important for the differentiation of muscle cells by binding, through its N-terminal domain, to the myocyte enhancer factor-2 (Mef2) protein, thus repressing expression of MEF2 downstream genes. Overexpression of HDAC5 in different cancer cells suppresses their growth by induction of apoptosis in a p53-independent manner. Reduced expression of the protein is observed in colon cancer and acute myeloid leukemia. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Histone Deacetylase 5 (HDAC5) (mouse IgG1 isotype) is derived from the HDAC5-35 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide. Mammalian HDACs can be divided into three classes according to sequence homology. Class II consists of the yeast Hda1-like proteins HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10. Histone deacetylase 5 (HDAC5) is a histone deacetylase, located on human chromosome 17q21. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| WGK Germany | - |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |