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Hydroxyphenyl fluorescein solution

SIGMA/H4290 - 5 mM in DMF

Synonym: Hydroxyphenyl fluorescein; 2-[6-(4′-hydroxy)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid; HPF

Empirical Formula (Hill Notation): C26H16O6
Molecular Weight: 424.40
EC Number: 200-679-5
Linear Formula: C26H16O6
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-H4290-1MG 1 mg
 $749.00
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concentration 5 mM in DMF
fluorescence λex 490 nm; λem 515 nm
Quality Level 200 
storage temp. 2-8°C
Application: Fluorescent reagent for the detection of highly reactive oxygen species (hROS). Fluorescence intensity increases after the immediate reaction with hROS such as hydroxyl radical and peroxynitrite. Since HPF does not react with nitric oxide, superoxide and hydrogen peroxide, it can differentially detect peroxynitrite. HPF is resistant to light-induced autoxidation.
Application: Hydroxyphenyl fluorescein solution is used to detect highly reactive oxygen species (hROS). It is a suitable probe to detect cellular death caused by bacterial antibiotics.
Features and Benefits: The following are the benefits of Hydroxyphenyl fluorescein solution:

• HPF is resistant to light-induced autooxidation.
• Hypochlorite(-OCl) does not react with HPF.
• HPF does not react with nitric oxide, superoxide, and hydrogen peroxide. 
• HPF has low reactivity with HOCl.
General description: Hydroxyphenyl fluorescein solution, also known as HPF is a fluorescent probe, which upon oxidation by the hydroxyl radical (HO) returns a brilliant green fluorescence (HPF-ox). Increased HPF signals in antibiotic-exposed bacterial cells are explained by fluorescence associated with increased cell size, not by ROS levels. In cells incubated with the HPF probe and exposed to ampicillin and norfloxacin with HPF fluorescence correlated linearly.
Storage Temp. 2-8°C
UNSPSC 12352108

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