HEPES

SIGMA/H4034 - BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

CAS Number: 7365-45-9
Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
Molecular Weight: 238.30
EC Number: 230-907-9
MDL Number: MFCD00006158
Linear Formula: C₈H₁₈N₂O₄S
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-H4034-25G	25 g	$62.30 1/EA	Add To Favorites
45-H4034-100G	100 g	$158.00 1/EA	Add To Favorites
45-H4034-500G	500 g	$552.00 1/EA	Add To Favorites
45-H4034-1KG	1 kg	$859.00 1/EA	Add To Favorites
45-H4034-25KG	25 kg	$6390.00 1/EA	Add To Favorites
45-H4034-25KG-DP	25 kg	No Price

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This image depicts SKU: H4034-100G

Properties
Descriptions
Safety Info.
Basic Data

absorption	≤0.05 at 260 in H₂O at 0.1 M
	≤0.05 at 280 in H₂O at 0.1 M
application(s)	clinical research diagnostic assay manufacturing general analytical life science and biopharma
assay	≥99.5% (titration)
cation traces	Fe: ≤5 ppm
color	white
foreign activity	DNase, RNase, NICKase, protease, none detected
form	crystalline powder
grade	BioPerformance Certified
impurities	endotoxin and total aerobic microbial count, tested
InChI	1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
pKa (25 °C)	7.5
Quality Level	400
SMILES string	OCCN1CCN(CC1)CCS(O)(=O)=O
storage condition	dry at room temperature
suitability	suitable for component for culture media
	suitable for Western blot
technique(s)	cell culture \| mammalian: suitable
	competitive inhibition ELISA: suitable
useful pH range	6.8-8.2

Application:	HEPES has been used: • To supplement Dulbecco’s modified Eagle’s medium to culture and maintain cell lines • As a component of platelet suspension buffer • To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue • As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay • For the adjustment and maintenance of pH of biological solutions • As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development • For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells • As a component of keratinocyte and fibroblast culture medium
Features and Benefits:	• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications • Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C • Negligible metal ion binding • Less toxic to cells than other buffers such as Tris and phosphate • Stable in a wide pH range • Tested for Endotoxins and Total Aerobic Microbial Count • Free from DNase, NICKase, RNase, Endonuclease, Exonuclease and Protease
General description:	HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures.
General description:	HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
General description:	HEPES, or 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, is a zwitterionic N-substituted aminosulfonic acid buffer. It demonstrates outstanding buffering capacity in the pH range of 6.8 to 8.2, boasting a pKa of 7.48 at 25°C. Widely acknowledged as one of the most versatile buffers, HEPES is extensively utilized in cell biology, biochemical, and biological research. A notable attribute is its non-reactivity with metal ions. Unlike many buffers, HEPES does not form significant complexes with most metals, making it suitable for applications involving metal ions without affecting their activity. This characteristic enhances its utility as a "non-coordinating buffer." In cell culture, HEPES excels in maintaining a stable physiological pH, even amidst fluctuations in carbon dioxide concentration resulting from cellular respiration. This sets it apart from bicarbonate buffers (NaHCO3), which, though commonly used, are less effective in pH stability. HEPES also proves advantageous in various biological and biochemical processes. Its ampholytic nature makes it suitable as a separator for creating pH gradients in isoelectric focusing, a technique often employed in protein separation and analysis. Moreover, HEPES exhibits minimal interference with DNA-restriction enzyme reactions compared to buffers with fewer substituted amine groups, such as Tris. This makes it a preferred choice for applications involving DNA manipulation and analysis. Beyond these specific applications, HEPES is valuable in numerous other biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging. Its versatility, coupled with exceptional pH buffering capacity and minimal interaction with other molecules, establishes HEPES as an indispensable tool across diverse research domains.
Legal Information:	DRYPOUR is a registered trademark of Merck KGaA, Darmstadt, Germany
Legal Information:	Tyvek is a registered trademark of E. I. du Pont de Nemours and Company
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	1 kg in poly bottle
Packaging:	100, 500 g in poly bottle
Packaging:	25 kg in poly drum
Packaging:	The DRYPOUR^® packaging system for high-quality hygroscopic salts DRYPOUR^® packaging:• The current DRYPOUR^® packaging configuration is in a 50L nestable drum • 25KG-DP are the standard prepack offering • Smaller volumes can be put in the same drum for variable configurations The benefits of DRYPOUR^®:• Improves operational efficiency by reducing material preparation time and increasing operator safety • Minimizes caking of hygroscopic salts • Offers triple protection: against moisture inside and outside, and against contamination How does the DRYPOUR^® packaging system Improve your operational efficiency?• This state-of-the-art design packaging system minimizes caking, thus significantly reducing the time needed for material preparation • PE drum with our new tamper-evident seal, a polyethylene liner with integrated desiccant bags and a breathable interior Tyvek^®liner • Desiccant bags are firmly integrated into the polyethylene liner • Triple protection delivers two invaluable results: Dramatically reduced caking No contamination risk from the desiccant

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99.5% (titration)
UNSPSC	12161700

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