HEPES

SIGMA/H3375 - ≥99.5% (titration)

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

CAS Number: 7365-45-9
Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
Molecular Weight: 238.30
EC Number: 230-907-9
MDL Number: MFCD00006158
Linear Formula: C₈H₁₈N₂O₄S
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-H3375-25G	25 g	$59.50 1/EA	Add To Favorites
45-H3375-100G	100 g	$110.00 1/EA	Add To Favorites
45-H3375-250G	250 g	$253.00 1/EA	Add To Favorites
45-H3375-500G	500 g	$458.00 1/EA	Add To Favorites
45-H3375-1KG	1 kg	$837.00 1/EA	Add To Favorites
45-H3375-5KG	5 kg	$2790.00 1/EA	Add To Favorites
45-H3375-25KG	25 kg	$6100.00 1/EA	Add To Favorites
45-H3375-50KG	50 kg	$11500.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: H3375-100G

Properties
Descriptions
Safety Info.
Basic Data

application(s)	diagnostic assay manufacturing general analytical life science and biopharma pharmaceutical
assay	≥99.5% (titration)
color	white
form	crystalline powder
InChI	1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
pH	5.0-6.5 (25 °C, 238 g/L)
pKa (25 °C)	7.5
Quality Level	400
SMILES string	OCCN1CCN(CC1)CCS(O)(=O)=O
solubility	water: 500 mg/mL, clear, colorless
storage condition	dry at room temperature
suitability	suitable for component for culture media
	suitable for enzyme extraction
technique(s)	cell culture \| mammalian: suitable
useful pH range	6.8-8.2

Application:	HEPES has been used: • As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo • As a component of Hanks’ solution used in the degradation testing of Iron • As a component of HEPES medium in the transfection of cell culture with plasmids • Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells • As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies
Features and Benefits:	• High purity product for biochemical and biological research • Suitable for Cell culture • Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
General description:	HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
General description:	HEPES, also known as 4-(2-Hydroxyethyl)piperazine-1-ethane-sulfonic acid, is a zwitterionic N-substituted aminosulfonic acid buffer. It is effective in the pH 6.8-8.2 range, with a pKa of 7.48 at 25°C. Recognized as one of the best all-purpose buffers, especially in cell biology, biochemical, and biological research, HEPES is a dipolar ionic buffer and one of the Good′s buffers that does not form significant complexes with most metal ions. This property makes it suitable as a non-coordinating buffer in solutions with metal ions. Widely utilized in cell culture, HEPES excels at maintaining physiological pH despite fluctuations in carbon dioxide concentration produced by cellular respiration, a feature not as prominent in bicarbonate buffers. It serves as an ampholytic separator for creating pH gradients in isoelectric focusing and exhibits less interference with DNA-restriction enzyme reactions compared to buffers with fewer substituted amine groups, such as Tris. Beyond these applications, HEPES may find application in various biological and biochemical studies, including immunoprecipitation, cell lysis, and live cell imaging. Its versatility makes it an indispensable tool in diverse research applications.
Legal Information:	Redi-Dri is a trademark of Sigma-Aldrich Co. LLC
Other Notes:	Go to Redi-Dri^™ Product Listing RDD002 Easily compare specifications for HEPES products with the HEPES specification table .
Other Notes:	2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	1 kg in poly bottle
Packaging:	25, 100, 250, 500 g in poly bottle
Packaging:	5, 25, 50 kg in poly drum

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99.5% (titration)
UNSPSC	12161700

To Order From a Quote