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HABA/Avidin Reagent

SIGMA/H2153 - lyophilized powder

Synonym: HABA Reagent

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-H2153-1VL 1 vial
$66.30
1/EA
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color orange
form lyophilized powder
Quality Level 200 
storage temp. 2-8°C
suitability suitable for spectrophotometric determination of biotin and biotin conjugates in a wide variety of samples
usage sufficient for ≤10 assays
Application: HABA/Avidin Reagent is suitable for spectrophotometric determination of biotin and biotin conjugates in a wide variety of samples.
Application: Reagent is capable of detecting biotin at μMolar concentrations (i.e., 1 nmole per ml). Typically used to quantitate biotin in biotinylated compounds.
Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description: 4-Hydroxyazobenzene-2-carboxylic acid (HABA) is an avidin-binding dye that can be stoichiometrically displaced by biotin. The subsequent change in absorption at A500 provides a basis for spectrophotometric determination of biotin levels in biological samples.
General description: HABA/Avidin Reagent is specific for chromogenic applications that depend upon quantification of biotin.
Preparation Note: Prepares a ready-to-use HABA/avidin solution in buffered saline, pH approx. 7.3, with preservative when reconstituted with 10 ml deionized water.
Hazard statements H412
Precautionary statements P273 - P501
Hazard Codes Xn
Risk Statements 21/22-52/53
Safety Statements 36/37-61
RIDADR NONH for all modes of transport
WGK Germany WGK 2
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. 2-8°C
UNSPSC 12352203

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