Hyaluronidase from Streptomyces hyalurolyticus
SIGMA/H1136
Synonym: Hyaluronate Lyase from Streptomyces hyalurolyticus
CAS Number: 37259-53-3
EC Number: 253-430-8
MDL Number: MFCD00131347
Product Type: Chemical
Hyaluronidase specificity: Hyaluronic acid is a polymer of alternating residues of β-D-(1-3) glucuronic acid and β-D-(1-4)-N-acetylglucosamine.
| biological source | Streptomyces sp. (S. hyalurolyticus) |
| conjugate | conjugate (Glucosaminoglycan) |
| form | film (Clear) |
| packaging | ampule of 300 × units |
| Quality Level | 200  |
| storage temp. | −20°C |
| Application: | Hyaluronidase from Streptomyces hyalurolyticus has been used: • in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue • as a component of digestion solution for the derivation of germline stem cells from testicular tissue |
| Biochem/physiol Actions: | Hyaluronate lyase cleaves hyaluronic acid at the β- |
| Biochem/physiol Actions: | Hyaluronidase is an endoglycosidase and performs its function along with exoglycosidases, like β-glucuronidase and β-N-acetyl glucosaminidase. Hyaluronidase regulates the hyaluronic acid turnover. |
| General description: | Hyaluronidase is widely present in nature. In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards. |
| Other Notes: | One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes). |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 4.2.2.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |