Monoclonal Anti-polyHistidine antibody produced in mouse
SIGMA/H1029 - clone HIS-1, ascites fluid
Synonym: Monoclonal Anti-polyHistidine; Monoclonal 6 His epitope tag; Monoclonal 6xHis-tag; Monoclonal HHHHHH epitope tag; Monoclonal Hexa His tag; Monoclonal His-tag; Monoclonal His6 tag; Monoclonal Histidine tagged; Monoclonal Poly-His-tag
Product Type: Chemical
Western Blotting JOURNAL CITATION: Short-chain fluorescent tryptophan tags for on-line detection of functional recombinant proteins. By: Siepert, E. M., Gartz, E., et al. in BMC Biotechnol, 2012. PubMed ID: 22999206 Image collected and cropped by CiteAb from the following publication, (http://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-12-65), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: TOX4 and NOVA1 proteins are partners of the LEDGF PWWP domain and affect HIV-1 replication. By: Morchikh, M., Naughtin, M., et al. in PLoS One, 2013. PubMed ID: 24312278 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0081217), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Activity-Regulated Cytoskeleton-Associated Protein Controls AMPAR Endocytosis through a Direct Interaction with Clathrin-Adaptor Protein 2. By: daSilva, L. L., Wall, M. J., et al. in Eneuro, 2016. PubMed ID: 27257628 Image collected and cropped by CiteAb from the following publication, (http://eneuro.sfn.org/cgi/doi/10.1523/ENEURO.0144-15.2016), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence HEK-293 cells overexpressing C-terminal polyHistidine tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1:2,000 Monoclonal Anti-polyHistidine Clone: HIS-1 (Cat. No. H1029). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Whole extract of human HEK-293T cells overexpressing C-terminal polyHistidine tagged fusion protein was separated on SDS-PAGE and probed with Monoclonal Anti-polyHistidine Clone: HIS-1 (Cat. No. H1029). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:500 2. Antibody dilution 1:1,000 3. Antibody dilution 1:3,000
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HIS-1, monoclonal |
| conjugate | unconjugated |
| form | liquid |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein |
| Application: | Antibody suitable for immunoblotting, dot blot assays, ELISA and immunocytochemistry. Monoclonal Anti-polyHistidine antibody produced in mouse has been used in Western blotting. |
| Biochem/physiol Actions: | The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors. |
| Biochem/physiol Actions: | The IgG2a (Immunoglobulin G 2a) gene actively takes part in stimulating autoimmunity in mice. The gene is found to be highly expressed in virus infection. |
| General description: | The Monoclonal Anti-polyHistidine (mouse IgG2a) antibody recognizes native or denatured, reduced forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The antibody preferentially recognizes the N-terminal tagged fusion protein and is reactive with fusion proteins expressed by the prokaryotic expression vectors pET, pRSET, and pTrc. |
| Immunogen: | recombinant polyHistidine tagged fusion protein. |
| Legal Information: | This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany. |
| Other Notes: | Recommended dilution for immunoblotting is 1:3000 |
| Physical form: | Suppled as ascites fluid with 15mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |