Superdex® 200 Increase 5/150 GL
SIGMA/GE28-9909-45 - Cytiva 28-9909-45, column L × I.D. 15 cm × 5 mm, 8.6 μm particle size
Synonym: Size-exclusion chromatography column
Product Type: Chemical
| bed volume | ~3 mL |
| cleaning | 1-14 |
| column L × I.D. | 15 cm × 5 mm |
| crosslinking | cross-linked |
| form | prepacked column |
| manufacturer/tradename | Cytiva 28-9909-45 |
| material | glass column |
| polyethylene filter | |
| matrix | agarose dextran composite |
| matrix active group | agarose and dextrans composite |
| mol wt | Mp 1-100 kDa (dextrans fractionation range) |
| Mr 1.3 MDa (exclusion limit) | |
| protein Mr 10-600 kDa (fractionation range) | |
| packaging | 1 ea of |
| parameter | 0.45 mL/min flow rate |
| 0.75 mL/min max. flow rate | |
| 3.0 Mpa max. pressure (725 psi) | |
| 4-50 μL sample volume | |
| particle size | 8.6 μm |
| product line | Superdex® |
| separation technique | size exclusion (SEC) |
| shipped in | ambient |
| storage condition | (20% ethanol ) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 3-12 |
| Application: | Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride |
| Application: | Well suited for rapid purity checks and quick screenings of experimental conditions. |
| Features and Benefits: | • Enhanced performance: improved resolution and runtime compared to predecessor Superdex™ 200. • Very high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity. • High flow rates: rigid beads give excellent pressure/flow properties. |
| General description: | Superdex™ 200 Increase size exclusion chromatography columns are well suited for high-resolution analysis and small-scale purification of monoclonal antibodies (mAb) and other biomolecules with Mr~ 10 000 to ~ 600 000. Superdex™ 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex™ Increase columns deliver higher resolution purification in shorter run times. Superdex™ 200 Increase has a selectivity curve optimized for excellent resolution of antibodies and macromolecules within the range Mr ~ 100 000 to ~ 300 000. It is well suited for detection and separation of antibody monomers, dimers, and aggregates present in monoclonal antibody preparations. The outstanding resolving power of this resin facilitates protein characterization, including analysis of antibody size variants, assessment of membrane protein size homogeneity, and the study of protein-protein interactions. Superdex™ 200 Increase is optimized to give high resolution separation for proteins with molecular weights in the range Mr ~ 100 000 to ~ 300 000. Superose™ 6 Increase provides a complementary fractionation range, resolving very large protein complexes and macromolecules that Superdex 200 Increase cannot separate. This agarose-based resin is alkali-resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples. |
| Legal Information: | Superdex is a registered trademark of Cytiva |
| Other Notes: | Discover LiChropur reagents ideal for HPLC  or LC-MS analysis |
| Preparation Note: | Storage Temp.: 4°C to 40°C |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN 1993 / PGIII |
| Storage Temp. | 2-30°C |
| UNSPSC | 41115700 |