HiLoad® 16/600 Superdex® 200 pg
SIGMA/GE28-9893-35 - Cytiva 28-9893-35, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size
Synonym: 16/600 Superdex 200; HiLoad Superdex 200
Product Type: Chemical
| avg. part. size | 34 μm |
| bed volume | 120 mL |
| cleaning | 1-14 |
| column L × I.D. | 60 cm × 16 mm |
| crosslinking | cross-linked |
| form | prepacked column |
| manufacturer/tradename | Cytiva 28-9893-35 |
| material | bed support (polyamide) |
| fiberglass end pieces (polypropylene reinforced) | |
| glass column | |
| matrix | cross-linked agaorse, spherical |
| dextran-agarose particle platform | |
| matrix active group | agarose |
| dextran-agarose phase | |
| mol wt | Mr 1-100 kDa (dextrans fractionation range) |
| protein Mr 10-600 kDa (fractionation range) | |
| packaging | pkg of 1 ea |
| parameter | <5 mL sample volume |
| 0.3 Mpa max. pressure | |
| 0.3-1.6 mL/min flow rate (recommended) | |
| separation technique | size exclusion (SEC) |
| shipped in | ambient |
| storage condition | (20% Ethanol ) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 3-12 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile |
| Application: | for high-resolution preparative gel filtration chromatography, e.g, separate mAbs from dimers and contaminants |
| Features and Benefits: | • High resolution with short run times and good recovery. • High capacity at high flow rates. • Separations can be scaled up to production levels. • Prepacked for convenience and reproducibility. • 1/16” fittings for convenient connections to AKTA design systems. |
| General description: | HiLoad® 16/600 Superdex® 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice for separating MAbs from dimers and contaminants. Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions. |
| Legal Information: | HiLoad is a registered trademark of Cytiva |
| Legal Information: | Superdex is a registered trademark of Cytiva |
| Other Notes: | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
| Preparation Note: | Storage Temp.: 4°C to 30°C |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-30°C |
| UNSPSC | 41115700 |