rProtein A/Protein G GraviTrap™
SIGMA/GE28-9852-56 - Cytiva 28-9852-56, pack of 10 × 1 mL
Synonym: antibody purification columns; chromatography columns; gravimetric columns; prepacked columns; rProtein A/G Sepharose FF
Product Type: Chemical
| bed volume | 1 mL |
| capacity | 35 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.) |
| manufacturer/tradename | Cytiva 28-9852-56 |
| packaging | pack of 10 × 1 mL |
| storage condition | (20% Ethanol) |
| storage temp. | 2-8°C |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | rProtein A Protein G GraviTrap™ are prepacked gravity-fLow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on Protein A and Protein G ligands in gravity-fLow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times. |
| Features and Benefits: | • Prepacked gravity-fLow columns provide convenience, save time, and increase the reliability of your results. • Purification of antibodies in 30 min allows you to quickly obtain and store your target Protein in order to avoid the inherent dangers of Protein instability and degradation associated with sLow and long purification runs. • High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%. • High binding capacity such as Protein A/Protein G GraviTrap™ ~ 35 mg human IgG/column. |
| General description: | rProtein A Protein G GraviTrap™ includes ten ready-to-use columns prepacked with 1 mL of a mixture of 50% rProtein A Sepharose™ Fast FLow and 50% protein G Sepharose™ 4 Fast Flow. |
| Legal Information: | GraviTrap is a trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 23151816 |