HiScreen™, DEAE Fast Flow
SIGMA/GE28-9782-45 - Cytiva 28-9782-45
Product Type: Chemical
| average diameter | 90 μm |
| bed size | 7.7 mm × 100 mm |
| bed volume | 4.7 mL |
| capacity | binding capacity(~ 110 mg HAS/mL resin) |
| cleaning | 2-14 |
| column I.D. | 7.7 mm |
| crosslinking | cross-linked |
| description | Ion Exchanger Type (value) |
| form | prepacked column |
| ligand | diethylaminoethyl |
| manufacturer/tradename | Cytiva 28-9782-45 |
| matrix | 6% cross-linked agarose |
| matrix active group | N+(C2H5)2H functional group |
| packaging | pkg of 1 ea |
| parameter | 1.5 bar (22 psi) (Over the Packed Bed During Operation) |
| 22 psi | |
| particle size | 45-165 μm |
| separation technique | anion exchange |
| storage condition | (20% Ethanol) |
| suitability | suitable for bioprocess medium |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 2-12 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | The small column volume of 4.7 mL and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Media prepacked in HiScreen™ IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production. |
| Features and Benefits: | • DEAE Sepharose Fast FLow is a weak anion exchanger and IEX Sepharose™ Fast FLow medium are well-proven, industrial standards for ion exchange chromatography. • Prepacked, ready-to-use columns for convenient process development optimization. • 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening. • Easily connected in series to achieve 20 cm bed height. • Small bed volume gives fast results and minimal sample/buffer consumption. • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity. |
| General description: | HiScreen™ DEAE FF columns are prepacked with DEAE Sepharose Fast Flow, a weak anion exchanger, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening. |
| Legal Information: | HiScreen is a trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| UNSPSC | 47101511 |