GST GraviTrap™
SIGMA/GE28-9523-60 - Cytiva 28-9523-60
Synonym: GST columns; affinity columns; chromatography columns; glutathione Sepharose 4B; gravity columns
Product Type: Chemical
| average diameter | 90 μm |
| bed volume | 2 mL |
| capacity | ≥20 mg binding capacity(recombinant glutathione S-transferase (rGST)) |
| >25 mg binding capacity (horse liver GST/ml medium) | |
| cleaning | 4-13 |
| column I.D. | 15 mm |
| ligand | glutathione |
| manufacturer/tradename | Cytiva 28-9523-60 |
| matrix | 4% agarose |
| packaging | pkg of 10 ea |
| particle size | 45-165 μm |
| working range | 4-13 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | GST GraviTrap™ contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose™ 4B, sufficient for purification of up to 20 mg of fusion Protein. GST-tagged Proteins are recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the Proteins. The binding capacity of prepacked GST GraviTrap™ columns, which can be greater than 10 mg Protein/mL medium, varies with the nature of the tagged Protein and the exact binding conditions used. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged Proteins. |
| Features and Benefits: | • Convenient, prepacked gravity-fLow columns. • Allows direct purification of both clarified and unclarified cell lysates. • Yields up to 20 mg of pure Proteins per column. • Yields up to 20 mg of pure Proteins per column |
| General description: | Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution. |
| General description: | GST GraviTrap™ contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose™ 4B, sufficient for purification of up to 20 mg of fusion protein. |
| Legal Information: | GraviTrap is a trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| UNSPSC | 41106501 |