GST Spintrap™
SIGMA/GE28-9523-59 - Cytiva 28-9523-59
Synonym: GST affinity columns; GST-tagged purification columns; chromatography columns; glutathione Stepharose 4B; spin columns
Product Type: Chemical
| average diameter | 90 μm |
| bed volume | 50 μL |
| capacity | ≥500 μg binding capacity(recombinant glutathione S-transferase (rGST)) |
| >25 mg binding capacity (horse liver GST/ml medium) | |
| cleaning | 4-13 |
| ligand | glutathione |
| manufacturer/tradename | Cytiva 28-9523-59 |
| matrix | 4% agarose |
| packaging | pkg of 50 ea |
| particle size | 45-165 μm |
| working range | 4-13 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • Preserves protein antigenicity and function. • Yields up to 500 μg of pure protein per column. • Allows direct purification of both clarified and unclarified cell lysates. |
| General description: | Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution. |
| General description: | GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates. GST-tagged proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves the functionality of the proteins, and more than 90% purity can be obtained in a single step. GST SpinTrap columns are suitable for purification of multiple samples in parallel, for example in screening experiments such as evaluation of chromatography media, growth temperature, culture density, and other variables to find optimal conditions for expression. |
| Legal Information: | SpinTrap is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (PBS and 0,05% Kathon CG/ICP Biocide) |
| RIDADR | NONH for all modes of transport |
| UNSPSC | 41106500 |