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Protein A Mag Sepharose™

SIGMA/GE28-9513-78 - Cytiva 28-9513-78, pack of 4 × 500 μL

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-GE28-9513-78 1 pkg
 $197.00
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capacity 8- 17 mg binding capacity(human IgG/ml gel)
ligand native protein A
manufacturer/tradename Cytiva 28-9513-78
matrix paramagnetic, spherical, highly cross-linked agarose particles
packaging pack of 4 × 500 μL
parameter Room temperature temp. range
storage condition (20% Ethanol, 20% medium slurry)
storage temp. 2-8°C
Analysis Note: To view the Certificate of Analysis for this product, please visit www.cytiva.com .
Application: Protein A Mag Sepharose™ are magnetic beads designed to simplify enrichment of target Proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose™ with native Protein A as ligand. Protein A has high affinity for the Fc region of IgG antibodies from a broad variety of species. There are two protocols available for Protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis. Protein A Mag Sepharose™ combines well-proven enrichment methods with the magnetic beads platform, which is highly suitable for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.
Features and Benefits: • Visible and dense Sepharose™ based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein
• Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
• Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)
• Optimized capacity for enrichment/immunoprecipitation of target Proteins - only Low amounts of antibody needed
• Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis
General description: protein A Mag Sepharose™ are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. Based on Sepharose™ with native protein A as ligand.
Legal Information: Sepharose is a trademark of Cytiva
Preparation Note: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Storage Temp. 2-8°C
UNSPSC 41106500

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