AutoSeq™ G-50
SIGMA/GE27-5340-01 - Cytiva 27-5340-01, pack of 50 columns
Synonym: PCR cleanup column; Sephadex; dye separator; resin; sequencing prep; solid phase extraction
Product Type: Chemical
| manufacturer/tradename | Cytiva 27-5340-01 |
| packaging | pack of 50 columns |
| particle size | 50-150 μm (dry) |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • For rapid removal of unincorporated fluorescent dye-terminators from cycle sequencing reactions prior to automated DNA sequencing • Columns are prepacked with Sephadex G-50 DNA Grade F • Columns are pre-equilibrated in double-distilled water with 0.05% Kathon CG/ICP Biocide added as a preservative • Ready-to-use columns enable sample application through to collection of purified product in less than 4 min • 4 minutes |
| General description: | AutoSeq G-50 columns are specifically designed to remove fluorescent dye-terminators from cycle sequencing reactions prior to analysis on automated sequencers. Effective DNA purification is essential for high-quality results on automated DNA sequencing since residual dye-terminators can obscure data. AutoSeq G-50 consists of MicroSpin columns containing Sephadex G-50 DNA Grade F, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artefacts on salt-sensitive automated DNA sequencers. |
| Legal Information: | AutoSeq is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| RIDADR | NONH for all modes of transport |
| UNSPSC | 41105501 |