Microspin™ S-400 HR
SIGMA/GE27-5140-01 - Cytiva 27-5140-01, pack of 50 columns
Synonym: Amersham spin column; DNA purification columns; Microspin S-400 HR; PCR cleanup columns; size exclusion columns; spin column
Product Type: Chemical
| avg. part. size | 50 μm |
| cleaning | 2-13 |
| manufacturer/tradename | Cytiva 27-5140-01 |
| material | resin (Sephacryl® S-400 HR) |
| matrix | allyl dextran and N, N’-methylenebisacrylamide |
| packaging | pack of 50 columns |
| particle size | 25-75 μm |
| sterility | non-sterile |
| storage condition | (20% Ethanol) |
| (TE buffer (10 mM Tris/HCl, 1mM EDTA, pH 7.6)) | |
| storage temp. | 2-8°C |
| suitability | suitable for bioprocess medium |
| working range | 3-11 |
| working volume | 25—100 μL |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • illustra™ MicroSpin™ S-400 HR Columns: For rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides using spin-column chromatography. - Accommodates 25 to 50 μ for post-PCR cleanup prior to cloning or a second amplification reaction or 51 to 100 μ for all other applications. • Convenient: Prepacked with Sephacryl® S-400 HR pre-equilibriated in TE buffer. • Ready to use: Requires less than 4 min from sample application to collection of purified product. • Tested in nickase, single- and double-stranded exonuclease and RNase assays. |
| General description: | MicroSpin™ columns are designed for the rapid purification of nucleic acids for use in a wide range of applications, including desalting, buffer exchange and removal of primers. Good product yield and purity is obtained with sample volumes from 25-100 μl, and from nanogram to milligram quantities of DNA. |
| Legal Information: | illustra is a trademark of Cytiva |
| Legal Information: | MicroSpin is a trademark of Cytiva |
| Legal Information: | Sephacryl is a registered trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 41105501 |