PreScission Protease
SIGMA/GE27-0843-01 - Cytiva 27-0843-01
Synonym: fusion protein, GST-tag removal
Product Type: Chemical
| manufacturer/tradename | Cytiva 27-0843-01 |
| packaging | pkg of 500 units |
| shipped in | dry ice |
| storage temp. | −70°C |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | Specific, low temperature cleavage of fusion protein tags; Simple on-column cleavage during affinity purification PreScission Protease has been used for eluting GST-tagged AT3, during in vitro ubiquitination reactions and AT3-Ub preparation. It has also been used to cleave GST tag from the a-enolase during induction of GST a-enolase expression in the protease-deficient BL21 strain of Escherichia coli. |
| Features and Benefits: | • Specific cleavage – between the Gln and Gly residues of the recognition sequence LeuGluValLeuPheGln/GlyPro • Time-saving – GST-tagged proteins can be cleaved while still bound to an affinity resin. • Low incubation temperature – enables low temperature cleavage of fusion proteins containing the HRV 3C protease recognition sequence. |
| General description: | PreScission Protease is a fusion protein of human rhinovirus (HRV) 3C protease and GST. It allows for on-column cleavage of GST tags and protein purification in one step. On-column or off-column enzyme cleavage GST tags and protease can be removed in a single step while fusion proteins are still bound to Glutathione Sepharose 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow affinity resin. Another option is to elute the GST-tagged protein using reduced glutathione, cleave, then pass the sample over the same resin to remove the protease and tags. Unlike other common proteases, such as thrombin and Factor Xa, this GST-tagged fusion protein can be removed easily through binding to a resin with affinity for GST. Other non-recombinant proteases require an additional purification step with a different resin. Vectors encoding the HRV 3C protease recognition sequence pGEX-6P vectors pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 encode the recognition sequence for this 3C protease. |
| Legal Information: | pGEX Vectors pGEX Vectors are to be used for scientific investigation and research and for no other purpose whatsoever and a license for commercial use of the licensed products and the processes claimed in US patent 5,654,176 and equivalent patents and patent applications in other countries must be negotiated directly with Millipore Corp (formerly Chemicon International Inc) by the purchaser prior to such use. |
| Preparation Note: | Storage Condition: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 41106501 |