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Ready-to-go T4 DNA Ligase

SIGMA/GE27-0361-01 - Cytiva 27-0361-01, sufficient for 50 reactions

Synonym: DNA ligation enzyme; RTG ligase; T4 DNA ligase enzyme; ligation enzyme

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-GE27-0361-01 1 pkg
$1010.00
1/EA
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Cytiva-27-0361-01

 

manufacturer/tradename Cytiva 27-0361-01
usage sufficient for 50 reactions
Analysis Note: To view the Certificate of Analysis for this product, please visit www.cytiva.com .
Application: Applications include self-circularization of linear DNA prior to transformation, ligation of insert to vector, and ligation of synthetic linkers to blunt-ended DNA. Each predispensed, single-reaction, ambient-temperature-stable tube contains a glassified reaction mix which, when reconstituted to a final reaction volume of 20 μL, yields a solution of reaction buffer, T4 DNA ligase, and ATP.
Features and Benefits: • For covalently-linking DNA fragments in as little as 30 min.
• Single-dose, ambient-temperature-stable reaction mixes.
General description: Ready-To-Go T4 DNA Ligase. Catalyzes the formation of a phosphodiester bond between the 5′-phosphoryl group and the 3′-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction. Ligation of blunt-ended DNA is greatly stimulated by polyethylene glycol.
Legal Information: Ready-To-Go is a trademark of Cytiva
Preparation Note: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
RIDADR NONH for all modes of transport
UNSPSC 12352204

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