HiTrap® Protein L
SIGMA/GE17-5478-51 - Cytiva 17-5478-51, pack of 5 × 1 mL
Synonym: HiTrap Chromatography Column; Protein L Column
Product Type: Chemical
HiTrap Protein L (1 and 5 mL)
| avg. part. size | 85 μm (d₅₀v is the median particle size of the cumulative volume distribution) |
| column I.D. | 7 mm |
| ligand | recombinant protein L (E. coli), mammalian free |
| manufacturer/tradename | Cytiva 17-5478-51 |
| matrix | rigid, highly cross-linked agarose |
| packaging | pack of 5 × 1 mL |
| parameter | 5 bar |
| storage condition | (20% Ehtanol) |
| storage temp. | 2-8°C |
| suitability | suitable for bioprocess medium |
| working range | 2-10(pH interval where the medium can be operated without significant change in function.) |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | HiTrap® Protein L may be used to characterize human colorectal cancer MDR1/P-gp Fab and for the purification optimization of a single-chain variable fragment (scFv) against type 1 insulin-like growth factor receptor (IGF-1R). It may also be used for the purification of antigen-binding fragments (Fabs) and domain antibodies (dAbs). |
| Features and Benefits: | • Captures a wide range of antibody fragments • Allows a platform approach for the purification of antibody fragment • Offers low ligand leakage, high binding capacity, and selectivity for a broad range of antibody fragments • Reduces process time and amount of resin • Suitable for process development and small-scale purification • HiTrap® format easily connects to a syringe, peristaltic pump, or chromatographic equipment, such as an ÄKTA® system. |
| General description: | HiTrap™ Protein L columns are prepacked with Capto™ L affinity chromatography resin for purification of antibody fragments containing the kappa light chain. This resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains. |
| General description: | HiTrap® Protein L columns are packed with Capto™ L affinity chromatography resin. It facilitates the purification of antibody fragments comprising the κ light chain. Capto™ L resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which binds to the variable region of antibody κ light chains with strong affinity. This resin is an effective capture platform for antibody fragments having κ light chains due to its strong binding capacity and affinity to a wide spectrum of antibody fragments. |
| Legal Information: | ÄKTA is a registered trademark of Cytiva |
| Legal Information: | Capto is a trademark of Cytiva |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| Storage Temp. | 2-8°C |
| UNSPSC | 23151816 |