HiTrap® IGY Purification
SIGMA/GE17-5111-01 - Cytiva 17-5111-01, pack of 1 × 5 mL
Synonym: IgY purification column; affinity column; chromatography column; egg yolk chromatography column; prepacked column; protein G column
Product Type: Chemical
Cytiva-17-5111-01
| avg. part. size | 34 μm |
| bed size | 16 mm × 25 mm |
| bed volume | 5 mL |
| cleaning | 2-13 |
| column I.D. | 16 mm |
| ligand | 2-mercaptopyridine |
| manufacturer/tradename | Cytiva 17-5111-01 |
| matrix | highly cross-linked 6% agarose |
| packaging | pack of 1 × 5 mL |
| parameter | <20 mL/min flow rate |
| 42 psi | |
| storage condition | (20% Ethanol) |
| storage temp. | 2-30°C |
| working range | 3-11 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | HiTrap® IgY Purification HP is packed with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction. |
| Features and Benefits: | • Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series. • Packed with a thiophilic adsorption medium, the ligand, 2-merCaptopyridine is coupled to Sepharose™ High Performance. • Recovery and purity of IgY are excellent. • Can be operated with a liquid chromatography system such as ÄKTA® design. • The top and bottom frits are manufactured from porous polyethylene • Connectors for usage with different chromatography systems and other equipment are included |
| General description: | HiTrap® IgY Purification HP is a 5 mL column prepacked with Sepharose™ High Performance for fast and easy purification of IgY from egg yolk. |
| Legal Information: | ÄKTA is a registered trademark of Cytiva |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Other Notes: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| Storage Temp. | 2-30°C |
| UNSPSC | 23151816 |