HiTrap® SP Fast Flow
SIGMA/GE17-5054-01 - Cytiva 17-5054-01, pack of 5 × 1 mL
Synonym: IEX column; SP Sepharose FF; chromatography cartridge; ion exchange column; prepacked column; strong cation exchanger
Product Type: Chemical
HiTrap SP FF, 5 x 1 mL
| average diameter | 90 μm |
| bed size | 7 mm × 25 mm |
| bed volume | 1 mL |
| cleaning | 3-14 |
| column I.D. | 7 mm |
| description | Ion Exchanger Type (value) |
| ligand | sulphopropyl |
| manufacturer/tradename | Cytiva 17-5054-01 |
| matrix | 6% cross-linked agarose |
| packaging | pack of 5 × 1 mL |
| parameter | flow rate |
| 42 psi | |
| particle size | 45-165 μm |
| suitability | suitable for bioprocess medium |
| working range | 4-13 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | Q, SP, DEAE, and CM Sepharose™ Fast FLow are based on a robust, 6% highly cross-linked beaded Agarose matrix with good flow properties and high loading capacities. ANX Sepharose™ 4 Fast FLow (high sub) is based on 4% highly cross-linked beaded Agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass Proteins. |
| Features and Benefits: | • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series. • The industry standard for ion exchange chromatography. • High fLow rates and good scale-up potential. • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient. • Predictable scale-up |
| General description: | HiTrap® SP FF is prepacked with SP Sepharose™ Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions. |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol) |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| UNSPSC | 23151816 |