Ni Sepharose™ Excel
SIGMA/GE17-3712-02 - Cytiva 17-3712-02, pack of 100 mL
Synonym: Ni Sepharose Excel
Product Type: Chemical
| capacity | ≥10 mg binding capacity (histidine-tagged protein) |
| cleaning in place | 2-14 |
| crosslinking | cross-linked |
| form | resin |
| manufacturer/tradename | Cytiva 17-3712-02 |
| matrix | Highly cross-linked spherical agarose |
| matrix active group | Nickel |
| operating pH | 2-12 |
| packaging | pack of 100 mL |
| parameter | 600 cm/hr max. flow rate |
| particle size | ~90 μm |
| separation technique | affinity |
| storage condition | (20% ethanol) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • Versatile – suitable for eukaryotic samples, including insect and CHO cells, as well as bacterial samples such as E. coli. • Direct sample load – of eukaryotic cell culture supernatants, eliminating the need for pretreatment of samples that usually cause nickel stripping. • Retained binding capacity of up to 10 mg/mL resin. • Simplified sample handling – fewer steps needed compared with conventional workflow. • Flexible formats – prepacked and loose resin formats for optimized screening and preparative his-tag purification. |
| General description: | Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function. |
| General description: | Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6- tagged pure protein (Mr 43 000) in EX-CELL® 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent. |
| General description: | Ni Sepharose excel is a his-tagged protein purification resin for minimized Ni-leakage and maximized protein recovery when samples contain stripping agents. EDTA-resistant resin for IMAC chromatography: Ni Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24 h incubation in 10 mM EDTA. Because of this strong binding, samples that typically cause stripping of metal ions can be loaded directly onto the resin without time-consuming pretreatment. Therefore, this his tag purification resin is especially suitable for purifying histidine-tagged (his-tagged) proteins secreted into eukaryotic cell culture supernatants. His tag purification of large sample volumes: The flow properties of Ni Sepharose excel nickel resin make it well-suited to purification at a wide range of scales. This resin can handle loading of large sample volumes, so you can purify low concentrations of target proteins using manual or automated methods. Convenient prepacked columns: • HisTrap excel column: HiTrap format for convenient use with a syringe, a peristaltic pump, or a chromatography system. • His MagSepharose excel: Magnetic beads for small-scale purification and screening. They are appropriate for early stages of research when many parallel experiments are required. |
| Legal Information: | EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS09 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-30°C |
| UNSPSC | 41106500 |