HiTrap® Desalting Columns
SIGMA/GE17-1408-01 - Cytiva 17-1408-01, pack of 5 × 5 mL
Synonym: Purification Columns
Product Type: Chemical
HiTrap Desalting
| bed volume | 5 mL |
| cleaning | 2-13 |
| column I.D. × L | 16 mm × 25 mm |
| crosslinking | cross-linked |
| form | prepacked column |
| manufacturer/tradename | Cytiva 17-1408-01 |
| matrix | cross-linked dextran |
| matrix active group | dextran |
| mol wt | protein Mr 5 kDa (exclusion limit) |
| packaging | pack of 5 × 5 mL |
| parameter | 0.1-1.5 mL sample volume |
| 1-10 mL/min flow rate | |
| 15 mL/min max. flow rate | |
| 5 bar max. pressure | |
| particle size | 20-50 μm (dry) |
| separation technique | size exclusion (SEC) |
| shipped in | ambient |
| storage condition | (20% ethanol) |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 2-13 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | Desalting and buffer exchange with Sephadex® G-25. |
| Features and Benefits: | • Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series. • Replaces dialysis as the method of choice for desalting. • Desalting requires less than one minute due to the high rigidity of Sephadex® G-25 and the optimized packing technology. |
| General description: | HiTrap® Desalting is a ready-to-use 5 mL column, prepacked with Sephadex® G-25 Superfine, for fast and convenient desalting and buffer exchange. HiTrap® Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction. |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | Sephadex is a registered trademark of Cytiva |
| Other Notes: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| Storage Temp. | 2-30°C |
| UNSPSC | 23151816 |