Glutathione Sepharose™ 4B
SIGMA/GE17-0756-01 - Cytiva 17-0756-01, pack of 10 mL
Synonym: affinity chromatography resin, GST-tagged proteins purification,
Product Type: Chemical
Glutathione Sepharose 4B
| average diameter | 90 μm |
| capacity | >25 mg binding capacity (recombinant GST / ml) |
| cleaning in place | 4-13 |
| manufacturer/tradename | Cytiva 17-0756-01 |
| matrix | 4% agarose |
| matrix active group | glutathione |
| packaging | pack of 10 mL |
| parameter | <100 cm/hr flow rate (Sample loading) |
| 100—300 cm/hr flow rate (wash and elution) | |
| separation technique | affinity |
| shipped in | ambient |
| storage condition | ( |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 4-13 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality. |
| General description: | Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution. |
| General description: | Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins. GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity. |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-30°C |
| UNSPSC | 41106500 |