HiTrap® Blue High Performance
SIGMA/GE17-0413-01 - Cytiva 17-0413-01, pack of 1 × 5 mL
Synonym: Cibacron Blue F3G-A column; albumin affinity column; chromatography column; interferon affinity column; prepacked column
Product Type: Chemical
HiTrap Blue HP
| avg. part. size | 34 μm |
| bed size | 16 mm × 25 mm |
| bed volume | 5 mL |
| cleaning | 3-13 |
| column I.D. | 16 mm |
| ligand | Cibacron Blue F3G-A |
| manufacturer/tradename | Cytiva 17-0413-01 |
| packaging | pack of 1 × 5 mL |
| parameter | 42 psi |
| working range | 4-12 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Application: | Blue Sepharose™ High Performance is a group specific adsorbent with affinity for a wide variety of enzymes. The dye ligand, Cibacron Blue F3G-A, is covalently attached to the matrix via the triazine part of the dye molecule. Some Proteins interact biospecifically with the dye due to its structural similarity with nucleotide cofactors, while others, such as albumin and interferon, bind in a less specific manner by electrostatic and/or hydrophobic interactions with the aromatic anionic ligand. |
| Features and Benefits: | • Can be used for depletion of albumin from serum. • Cibacron Blue F3G-A, the ligand, is covalently attached to the highly cross-linked Agarose medium and is stable over a pH range of 4 to 12 for use and 3 to 13 for cleaning. • The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC™ System. |
| General description: | HiTrap® Blue HP is prepacked with Blue Sepharose™ High Performance for capture of a wide range of biomolecules, including: albumin, interferon, a broad range of nucleotide-dependent enzymes, α2-macroglobulin, coagulation factors, and related proteins. |
| Legal Information: | ÄKTA is a registered trademark of Cytiva |
| Legal Information: | FPLC is a trademark of Cytiva |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Other Notes: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| UNSPSC | 23151816 |