HiTrap® Protein A High Performance
SIGMA/GE17-0403-01 - Cytiva 17-0403-01, pack of 1 × 5 mL
Synonym: Protein A Affinity Column
Product Type: Chemical
| avg. part. size | 34 μm |
| bed volume | 5 mL |
| capacity | ~20 mg binding capacity (human IgG / mL resin) |
| cleaning | 2-10 |
| column I.D. × L | 16 mm × 25 mm |
| crosslinking | cross-linked |
| form | prepacked column |
| manufacturer/tradename | Cytiva 17-0403-01 |
| matrix | highly cross-linked agarose, spherical |
| matrix active group | Protein A |
| packaging | pack of 1 × 5 mL |
| parameter | 0.5 Mpa max. pressure |
| 20 mL/min max. flow rate | |
| 5 mL/min flow rate | |
| separation technique | affinity |
| storage condition | (20% Ethanol) |
| storage temp. | 2-8°C |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 3-9 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • High binding capacitiy of 20 mg IgG/mL medium and fast kinetics. • 34 μm bead size for high-performance, high-resolution purifications. • Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC™ System. • Stable over pH 3 to 9 for use, and 2 to 10 for cleaning. • Robust, easy-to-use method. |
| General description: | HiTrap® Protein A HP is a prepacked Protein A Sepharose™ High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants. HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format. |
| General description: | pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH. |
| Legal Information: | FPLC is a trademark of Cytiva |
| Legal Information: | HiTrap is a registered trademark of Cytiva |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Other Notes: | The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used. |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Symbol |  GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| Storage Temp. | 2-8°C |
| UNSPSC | 23151816 |