Ficoll® PM 400
SIGMA/GE17-0300-10 - solid, spray-dried, solubility:, soluble, non-sterile (endotoxins < 0.12 EU/mL), pack of 100 g
Synonym: 17-0300-10; Density gradient medium; cell culture media; cell purification media; cell storage media; lymphocyte separation medium; mononuclear cell isolation
Product Type: Chemical
| color | White |
| density | ≤1.2 g/mL |
| dilution | (Used to form density gradients for centrifugation seperation applications) |
| form | solid |
| spray-dried | |
| manufacturer/tradename | Cytiva 17-0300-10 |
| packaging | pack of 100 g |
| shelf life | 5 yr |
| solubility | soluble |
| sterility | non-sterile (endotoxins < 0.12 EU/mL) |
| storage temp. | 2-30°C |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com   . |
| Features and Benefits: | • For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity. • Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution. • Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved. • Better osmotic properties than sucrose. • Preserves functional and morphological integrity. • Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity). • Does not penetrate biological membranes. • Serves as the raw material for preparation of Ficoll-Paque gradients. • Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier. |
| General description: | High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. The molecules are highly branched and the high content of hydroxyl groups leads to very good solubility in aqueous media. |
| General description: | It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained |
| Legal Information: | Ficoll is a registered trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C () |
| RIDADR | NONH for all modes of transport |
| Density | ≤1.2 g/mL |
| Storage Temp. | 2-30°C |
| UNSPSC | 41105330 |