Sepharose™ CL-4B
SIGMA/GE17-0150-01 - Cytiva 17-0150-01, pack of 1 L
Synonym: chromatography resin; coupling matrix; crosslinked agarose resin; cytiva 17-0150-01; filtration gel; unactivated resin
CAS Number: 61970-08-9
MDL Number: MFCD00148279
Product Type: Chemical
| cleaning in place | 3-14 |
| crosslinking | cross-linked |
| feature | autoclavable |
| form | resin |
| manufacturer/tradename | Cytiva 17-0150-01 |
| matrix | 4% cross-linked agarose |
| matrix active group | agarose |
| operating pH | 3-13 |
| packaging | pack of 1 L |
| parameter | 26 cm/hr max. flow rate |
| particle size | 45-165 μm |
| pore size | 60-20000 kDa fractionation range (globular proteins) |
| separation technique | size exclusion (SEC) |
| storage condition | ( |
| storage temp. | 2-30°C |
| technique(s) | liquid chromatography (LC): suitable |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com ![]() |
| Features and Benefits: | • 4% cross-linked agarose gel filtration media. • Proven base matrix for coupling affinity ligands • Higher degree of crosslinking improve matrix stability |
| General description: | Sepharose™ CL-4B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling. Sepharose™ is a bead-formed agarose-based gel filtration matrix. Sepharose™ is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B respectively, Both Sepharose™ and Sepharose™ CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight. Sepharose™ CL gels are cross-linked derivatives of Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B. The cross-linked form of Sepharose™ is chemically and physically more resistant than Sepharose™ itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose™ gels are resistant to organic solvents and are thus the choice for separations in organic solvents. |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C (20% Ethanol) |
| Symbol | GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-30°C |
| UNSPSC | 41106500 |


