Sephadex® G-50
SIGMA/GE17-0042-01 - Fine, Cytiva, pack of 100 g
CAS Number: 9004-54-0
MDL Number: MFCD23701222.obs1210407
Product Type: Chemical
| cleaning in place | 2-13 |
| crosslinking | cross-linked |
| feature | autoclavable In wet form (pH 7.0) at 120°C for 30 min |
| form | resin |
| InChI | 1S/C18H32O16/c19-1-5(21)9 |
| InChI key | FZWBNHMXJMCXLU-UHFFFAOYSA |
| manufacturer/tradename | Cytiva |
| matrix | cross-linked dextran |
| matrix active group | dextran |
| mol wt | protein 3-20 kDa (fractionation range) |
| protein Mr 30 kDa (exclusion limit) | |
| packaging | pack of 100 g |
| particle size | 20-80 μm (dry) |
| 41-171 (wet) | |
| separation technique | size exclusion (SEC) |
| shipped in | ambient |
| SMILES string | O1C(C(C(C(C1CO)O)O)O)OCC2 |
| storage temp. | 2-30°C |
| swelling | 1 g swells to 13-16 mL |
| technique(s) | liquid chromatography (LC): suitable |
| working range | 2-12 |
| Analysis Note: | To view the Certificate of Analysis for this product, please visit www.cytiva.com ![]() |
| Features and Benefits: | • Quickly desalts, removes contaminants and transfers to a new buffer in a single step. • Suitable for purification of DNA from small molecules by gel filtration • Small bead size for shorter diffusion distances • Classic gel filtration medium. |
| General description: | Sephadex® G-50 Fine is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Fine´s small bead size give higher efficiency. Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations at where high flow rates and low operating pressures are required. |
| Legal Information: | Sephadex is a registered trademark of Cytiva |
| Preparation Note: | Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. |
| Preparation Note: | Store at 4 to 30 °C () |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-30°C |
| UNSPSC | 41115722 |

