β-Galactosidase Reporter Gene Staining Kit

SIGMA/GALS

Synonym: β-galactosidase; LacZ

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-GALS-1KT	1 kit	$400.00 1/EA		Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

Quality Level	200
shipped in	dry ice
storage temp.	−20°C
technique(s)	microbe id \| staining: suitable
usage	kit sufficient for 100 tests (using a 3.5 cm dish)

Application:	β-Galactosidase Reporter Gene Staining Kit has been used for histochemical staining of animal cells or tissues such as: • sagittal brain sections in histoenzymology • pancreas or colon sections in β-galactosidase reporter gene assay • mice E10.5 -11.5 embryo and embryo sections
General description:	β-galactosidase (β-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole. The functionality of β-gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide. The colorimetric detection methods also use other substrates of β-gal like O-nitrophenyl-β-D-galactopyranoside (ONPG). β-gal is a cost-effective, simple and standard reporter system. The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the β-galactosidase gene, LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.
Other Notes:	The substrate used in this kit is X-Gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) which results in an indigo blue-colored staining of transfected cells and tissues. This test provides a method for determining the percentage of transfected cells that are expressing LacZ or for microscopically visualizing reporter gene expression in tissue sections.

Symbol	GHS02,GHS05,GHS06,GHS08
Signal word	Danger
Hazard statements	H226 - H302 + H312 - H314 - H317 - H331 - H334 - H335 - H341 - H350 - H412
Precautionary statements	P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
Hazard Codes	C
Risk Statements	20/21/22-34-40-42/43
Safety Statements	23-26-36/37/39-45
RIDADR	UN 3316 9
WGK Germany	WGK 3
Flash Point(F)	132.8 °F
Flash Point(C)	56 °C
Supplemental Hazard Statements	EUH032

Storage Temp.	−20°C
UNSPSC	12161503

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