Anti-GAPDH antibody produced in rabbit

SIGMA/G9545 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti Gapdh; Anti Gapdh Antibody; Anti-Gapdh; GAPDH Antibody - Anti-GAPDH antibody produced in rabbit; Gapdh Antibody; Gapdh Antibody Sigma; Anti-38-kD component; Anti-G3PD; Anti-GAPD; Anti-Glyceraldehyde-3-phosphate dehydrogenase; Anti-OCAS; Anti-OCT1 coactivator in S phase; Anti-p38 component

MDL Number: MFCD01322099
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-G9545-100UL	100 µL	$515.00 1/EA	Add To Favorites
45-G9545-200UL	200 µL	$664.00 1/EA	Add To Favorites

Western Blotting JOURNAL CITATION: Proteasomal degradation of Mcl-1 by maritoclax induces apoptosis and enhances the efficacy of ABT-737 in melanoma cells. By: Pandey, M. K., Gowda, K., et al. in PLoS One, 2013. PubMed ID: 24223823 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0078570), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.

Western Blotting JOURNAL CITATION: The anti-migratory effects of FKBPL and its peptide derivative, AD-01: regulation of CD44 and the cytoskeletal pathway. By: Yakkundi, A., McCallum, L., et al. in PLoS One, 2013. PubMed ID: 23457460 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0055075), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.

Immunofluorescence NRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-GAPDH (Cat. No. G9545). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunoblotting Whole mouse 3T3 (A) or human HeLa (B) cell extracts were separated on SDS-PAGE and blotted with Rabbit Anti-GAPDH (Cat. No. G9545). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1: Antibody 0.1 μg/mL 2: Antibody 0.1 μg/mL + GAPDH immunizing peptide (mouse 314-333)

Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.2 μg/mL Rabbit Anti-GAPDH (Cat. No. G9545). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. PC-12 6. RAT2 7. CHO 8. MDBK 9. MDCK

Properties
Descriptions
Safety Info.
Basic Data

antibody form	affinity isolated antibody
antibody product type	primary antibodies
biological source	rabbit
clone	polyclonal
concentration	~1 mg/mL
conjugate	unconjugated
form	buffered aqueous solution
mol wt	antigen ~36 kDa
Quality Level	200
shipped in	dry ice
species reactivity	mouse, rat, human
storage temp.	−20°C
target post-translational modification	unmodified
technique(s)	immunoprecipitation (IP): 5-10 μg using mouse NIH3T3 cell lysates
	indirect immunofluorescence: 5-10 μg/mL using rat NRK cells
	western blot: 0.1-0.2 μg/mL using whole extract of human HeLa cells
UniProt accession no.	P04406

Application:	Anti-GAPDH antibody produced in rabbit is suitable as a primary antibody for western blotting using: • human cancer cell extract • extract from muscle tissue from old mice exhibiting sarcopenia • trabeculae or samples of the mice heart myocardium • mouse embryonic fibroblasts It is suitable for immunoprecipitation using mouse NIH3T3 cell lysates with 5-10μg, for indirect immunofluorescence using rat NRK cells at a working concentration of 5-10μg/mL. It is also suitable for western blotting at a working concentration of 0.1-0.2μg/mL using a whole extract of human HeLa cells.
Biochem/physiol Actions:	Anti-GAPDH recognizes human, mouse, and rat GAPDH.
Biochem/physiol Actions:	The enzyme glyceraldehyde-3-phosphate dehydrogenase catalyzes the reversible oxidative phosphorylation of glyceraldehyde-phosphate in the presence of inorganic phosphate and NAD, which is a critical energy-yielding step in carbohydrate metabolism. It binds to several proteins including actin, tubulin, amyloid precursor, polyglutamine peptides, DRPLA (dentatorubral-pallidoluysian atrophy), and huntingtin. GAPDH forms a part of OCA-S, the multicomponent OCT1 (octamer-motif-binding factor) coactivator complex, which is involved in the S phase-dependent histone H2B transcription. This association is responsible for linking H2B transcriptional machinery to cell cycle regulation and to the cellular metabolic state. In response to oxidative stress, GAPDH induces apoptosis.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	The GAPDH (glyceraldehyde-3-phosphate dehydrogenase) gene is mapped to human chromosome 12p13.3 and encodes a tetramer containing identical chains. The enzyme is commonly known as glycolytic enzyme and contains a binding site for NAD⁺(nicotinamide adenine diphosphate) and glyceraldehyde-3-phosphate. GAPDH contributes to 10-20% of the total cellular protein and is considered to be evolutionarily conserved.
Immunogen:	Synthetic peptide corresponding to amino acids of mouse GAPDH, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence is identical in rat and differs by two amino acids in humans.
Physical form:	Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note:	For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3

Storage Temp.	−20°C
UNSPSC	12352203

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