Anti-GAPDH antibody, Mouse monoclonal

SIGMA/G8795 - clone GAPDH-71.1, purified from hybridoma cell culture

Synonym: GAPDH Antibody - Monoclonal Anti-GAPDH antibody produced in mouse; Gapdh Antibody; Anti-G3PD; Anti-G3PDH; Anti-Glyceraldehyde-3-phosphate dehydrogenase; Loading Control

MDL Number: MFCD01322099
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-G8795-25UL	25 µL	DISCONTINUED
45-G8795-100UL	100 µL	$515.00 1/EA	Add To Favorites
45-G8795-200UL	200 µL	$679.00 1/EA	Add To Favorites

Western Blotting JOURNAL CITATION: Thyroid hormone enhances nitric oxide-mediated bacterial clearance and promotes survival after meningococcal infection. By: Chen, Y., Sjölinder, M., et al. in PLoS One, 2012. PubMed ID: 22844479 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0041445), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.

Western Blotting JOURNAL CITATION: Acetonic extract of Buxus sempervirens induces cell cycle arrest, apoptosis and autophagy in breast cancer cells. By: Ait-Mohamed, O., Battisti, V., et al. in PLoS One, 2011. PubMed ID: 21935420 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0024537), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.

Immunofluorescence NRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 5 μg/mL Monoclonal Anti-GAPDH Clone: GAPDH-71.1 (Cat. No. G8795). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunoblotting Total cell extract of A431 cells was separated on SDS-PAGE and blotted with Monoclonal Anti-GAPDH (Cat. No. G8795). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate (Cat. No. A2304), and a chemiluminescent substrate. Lanes: 1. 0.2 μg/mL antibody 2. 0.05 μg/mL antibody 3. 0.025 μg/mL antibody 4. 0.0125 μg/mL antibody 5. 0.01 μg/mL antibody

Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-GAPDH Clone: GAPDH-71.1 (Cat. No. G8795). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. PC-12 6. RAT2 7. CHO 8. MDBK 9. MDCK

Properties
Descriptions
Safety Info.
Basic Data

antibody form	purified from hybridoma cell culture
	purified immunoglobulin
antibody product type	primary antibodies
application(s)	research pathology
biological source	mouse
clone	GAPDH-71.1, monoclonal
concentration	~1 mg/mL
conjugate	unconjugated
form	buffered aqueous solution
isotype	IgM
mol wt	antigen ~37 kDa
packaging	antibody small pack of 25 μL
Quality Level	200
shipped in	dry ice
should not react with	prokaryotes
species reactivity	bovine, turkey, canine, chicken, monkey, mink, mouse, human, rabbit, rat, hamster
storage temp.	−20°C
target post-translational modification	unmodified
technique(s)	immunocytochemistry: suitable
	indirect ELISA: suitable
	microarray: suitable
	western blot: 0.025-0.05 μg/mL using A431 total cell extract
UniProt accession no.	P04406

Application:	Monoclonal Anti-GAPDH antibody produced in mouse is suitable for western blotting using: • protein extracted from heart tissue of mice at a working dilution of 1:25,000 • myelin and axogliasomal fractions from human CNS • nuclear and cytoplasmic fractions from TBP-13Q and TBP-105Q PC12 cells following recovery from heat shock • protein from bovine immortalized luteal endothelial cells • renal tubular epithelial cell extract • proteins from mouse embryonic fibroblasts • protein extract from ventricular myocardium tissues • A431 total cell extract at a working concentration of 0.025-0.05μg/mL It is also suitable for immunostaining using leiomyomas and leiomyosarcomas. The antibody can also be used for immunocytochemistry, indirect ELISA and microarray.
Biochem/physiol Actions:	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a tetramer containing identical chains. It catalyzes the reversible oxidative phosphorylation of glyceraldehyde-phosphate, which is a critical energy-yielding step in carbohydrate metabolism. It binds to several proteins including actin, tubulin, amyloid precursor, polyglutamine peptides, DRPLA (dentatorubral-pallidoluysian atrophy), and huntingtin. Protein kinase Cι/λbinds and phosphorylates GAPDH. Phosphorylated GAPDH associates with cytoskeletal elements and controls microtubule dynamics in the early secretory pathway. Poly(ADP-ribose) polymerase-1 (PARP1) interacts with GAPDH and thereby mediates brain damage in the presence of oxidative/nitrosative stress. GAPDH forms a part of OCA-S, the multicomponent OCT1 (octamer-motif-binding factor) coactivator complex, which is involved in the S phase-dependent histone H2B transcription. This association is responsible for linking H2B transcriptional machinery to cell cycle regulation and to the cellular metabolic state. GAPDH is also a component of the functional GAIT (interferon-γ-activated inhibitor of translation) mRNP (messenger ribonucleoprotein). GAPDH expression is dysregulated during melanoma progression.
Biochem/physiol Actions:	Monoclonal Anti-GAPDH recognizes human, monkey, bovine, canine, rat, mouse, hamster, mink, rabbit, chicken, and turkey GAPDH. It does not cross-react with non-vertebrate and prokaryotic species.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	Monoclonal Anti-GAPDH (mouse IgM isotype) is derived from the hybridoma GAPDH-71.1 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with rabbit GAPDH (glyceraldehyde-3-phosphate dehydrogenase). The gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is mapped to human chromosome 12p13. The protein localizes in the cytoplasm but can be translocated to the nucleus depending on cellular conditions.
Immunogen:	Rabbit GAPDH.
Other Notes:	2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Physical form:	Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note:	For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
UNSPSC	12352203

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