β-Glucuronidase from limpets (Patella vulgata)
SIGMA/G8132 - Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid
Synonym: β-D-Glucuronide glucuronosohydrolase
CAS Number: 9001-45-0
EC Number: 232-606-8
MDL Number: MFCD00130629
Product Type: Chemical
β-Glucuronidase is routinely used to hydrolyze glucuronide-conjugates of drug metabolites in order to optimize sample preparation prior to LC-MS, GC and ELISA analysis.
LC-MS analysis of codeine-glucuronide enzymatic hydrolysis with six different Sigma-produced β-glucuronidase preparations. The limpet-derived enzyme G8132 performed best with this substrate, with reaction completion within 3–4 hours at 60 °C at 1 unit per mL. Comparative data above was performed at 60 °C at 10 unit per mL.
LC-MS analysis of hydromorphone-glucuronide enzymatic hydrolysis with six different Sigma-produced β-glucuronidase preparations. Molluscan enzymes performed similarly. The reaction was complete within 3–4 hours at 60 °C at 1 unit per mL. The E. coli enzymes exhibited low specificity for this substrate.
Enzymatic hydrolysis rates of six glucuronide substrates at 1,000 ng/mL in synthetic urine using heavy-labeled internal standards at 250 ng/ml. Hydrolysis was performed at 10 units/mL α-glucuronidase in acetate buffer at pH 5.0.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G8132-100KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G8132-250KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G8132-500KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G8132-1MU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G8132-2MU
| biological source | limpet (Patella vulgata) |
| form | lyophilized powder |
| Quality Level | 300  |
| shipped in | wet ice |
| specific activity | 1,000,000-3,000,000 units |
| storage temp. | −20°C |
| type | Type L-II |
| Analysis Note: | Contains sulfatase activity which is inhibited by 0.1 M phosphate. |
| Application: | New Technical Article Comparing Performance of Different Enzymes Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine |
| Application: | The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes |
| Application: | β-Glucuronidase Type L-II has been shown to be a superior enzyme for the hydrolysis of drug-glucuronides from urine. |
| Biochem/physiol Actions: | β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption. Morphine 3-β-D glucuronide was converted to morphine using ß-glucuronidase obtained from Patella vulgate. |
| Other Notes: | One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay). |
| Packaging: | 100000, 250000, 500000, 1000000, 2000000 units in poly bottle |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P280 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 22-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 1,000,000-3,000,000 units |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.31 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |