Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit
SIGMA/G7781 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: The homeodomain transcription factor Hoxa2 interacts with and promotes the proteasomal degradation of the E3 ubiquitin protein ligase RCHY1. By: Bergiers, I., Bridoux, L., et al. in PLoS One, 2013. PubMed ID: 24244684 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0080387), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Identification of novel type III secretion chaperone-substrate complexes of Chlamydia trachomatis. By: Pais, S. V., Milho, C., et al. in PLoS One, 2013. PubMed ID: 23431368 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0056292), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence Human HEK-293T cells over-expressing GST tagged fusion protein were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:4,000 Rabbit Anti-Glutathione-S-Transferase (GST) (Cat. No. G7781). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting
Glutathione S-Transferase (GST) from E. coli (Cat. No. G5663) was separated on SDS-PAGE and probed with 1:2,000 Rabbit Anti-Glutathione-S-Transferase (GST) (Cat. No. G7781). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. GST 0.05 μg/lane
2. GST 0.025 μg/lane
3. GST 0.0125 μg/lane
4. GST 0.0062 μg/lane
Glutathione S-Transferase (GST) from E. coli (Cat. No. G5663) was separated on SDS-PAGE and probed with 1:2,000 Rabbit Anti-Glutathione-S-Transferase (GST) (Cat. No. G7781). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. GST 0.05 μg/lane
2. GST 0.025 μg/lane
3. GST 0.0125 μg/lane
4. GST 0.0062 μg/lane
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| indirect ELISA: 1:10,000 | |
| western blot: 1:2,000 using lysates of induced Escherichia coli expressing GST |
| Application: | Anti-Glutathione-S-Transf |
| Biochem/physiol Actions: | Glutathione-S-transferase (GST) catalyzes the detoxication of electrophiles by glutathione conjugation. This enzyme provides protection against oxidative stress and toxic foreign chemicals. Polymorphism in GST gene is associated with the development of asthma and cutaneous basal cell carcinoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Glutathione-S-Transf |
| General description: | The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins. |
| Immunogen: | recombinant GST from Schistosoma japonicum expressed in E. coli. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |