Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse
SIGMA/G6539 - clone GFP-20, ascites fluid
Synonym: GFP Antibody - Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse; Gfp Antibody Sigma; Gfp Monoclonal Antibody
MDL Number: MFCD01092266
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Selective disruption of aurora C kinase reveals distinct functions from aurora B kinase during meiosis in mouse oocytes. By: Balboula, A. Z. & Schindler, K. in PLoS Genet, 2014. PubMed ID: 24586209 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1004194), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Selective disruption of aurora C kinase reveals distinct functions from aurora B kinase during meiosis in mouse oocytes. By: Balboula, A. Z. & Schindler, K. in PLoS Genet, 2014. PubMed ID: 24586209 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1004194), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Hypoxia Inducible Factor 1 Alpha Is Expressed in Germ Cells throughout the Murine Life Cycle. By: Takahashi, N., Davy, P. M., et al. in PLoS One, 2016. PubMed ID: 27148974 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0154309), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence HEK-293 cells overexpressing GFP tagged fusion protein were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1:500 Monoclonal Anti-GFP Clone: GFP-20 (Cat. No. G6539). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Whole extract of human HEK-293T cells over-expressing N-Terminal GFP tagged fusion protein was separated on SDS-PAGE and probed with Monoclonal Anti-Green Fluorescent Protein (GFP), Clone: GFP-20 (Cat. No. G6539). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:2,000 2. Negative Control: without first antibody
Immunoblotting Whole extract of human HEK-293T cells over-expressing C-Terminal GFP tagged fusion protein was separated on SDS-PAGE and probed with Monoclonal Anti-Green Fluorescent Protein (GFP), Clone: GFP-20 (Cat. No. G6539). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:2,000 2. Negative Control: without first antibody
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GFP-20, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 27 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| indirect ELISA: suitable | |
| western blot: 1:2,000 using purified recombinant GFP preparation |
| Application: | Monoclonal Anti-Green Fluorescent Protein (GFP) antibody is suitable for use in western blot and ChIP assays. |
| Application: | Monoclonal Anti-Green Fluorescent Protein (GFP) recognizes GFP (27 kDa) using immunoblotting, dot blot, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation and enzyme linked immunosorbent assay (ELISA). The antibody reacts with fusion proteins expressed by prokaryotes expression vectors. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Green Fluorescent Protein (GFP) (mouse IgG1 isotype) is derived from the GFP-20 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a GFP tagged fusion protein. GFP is a 27 kDa (238 amino acid) protein, derived from the bioluminescent jellyfish Aequorea victoria, in which light is produced when energy is transferred from the Ca2+-activated photoprotein aequorin to GFP. GFP is a unique tool to monitor dynamic processes in a variety of living cells or organisms. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. Exogenous substrates and cofactors are not required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP signals can be quantified by flow cytometry, confocal scanning laser microscopy, and fluorometric assays. Indeed, many recombinant proteins have been engineered with GFP tags to facilitate the detection, isolation and purification of the proteins. |
| General description: | The Green Fluorescent Protein (GFP) is derived from the jellyfish Aequorea Victoria and is often used as a gene expression marker. Thus, antibodies to GFP can facilitate the detection and analysis of GFP labeled nucleic acids and proteins. Monoclonal Anti-Green Fluorescent Protein (GFP) antibody is specific for GFP and GFP-labeled biomolecules. |
| Immunogen: | GFP tagged fusion protein |
| Immunogen: | GFP tagged fusion protein. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |