Anti-GroEL antibody produced in rabbit
SIGMA/G6532 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-GroEL; GroEL Antibody - Anti-GroEL antibody produced in rabbit - Tested Applications: immunoblotting 1:80,000,indirect ELISA 1:40,000-1:80,000 - Sigma Aldrich G6532; Groel Antibody
Product Type: Chemical
Western Blotting JOURNAL CITATION: The chlamydial periplasmic stress response serine protease cHtrA is secreted into host cell cytosol. By: Wu, X., Lei, L., et al. in BMC Microbiol, 2011. PubMed ID: 21527029 Image collected and cropped by CiteAb from the following publication, (http://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-11-87), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting E. Coli Bacterial extract and E. Coli were separated on SDS-PAGE and probed with IGG FRAC TO GRO-EL (RB) (PREPACK) (Cat. No. G6532). The antibody was developed with HRP APA Rabbit IgG (Gt) (Cat. No. A0545), ECL and AEC substrate, respectively. Antibody dilutions 1/160,000 and 1/10,000, respectively.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | E. coli |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:40,000-1:80,000 |
| western blot: 1:80,000 |
| Application: | Anti-GroEL antibody produced in rabbit has been used as positive control in histology and immunohistochemistry of Dirofilaria immitis, nematode worms and in the western blot analysis of the HeLa cells. |
| Biochem/physiol Actions: | GroEL, together with GroES, in the presence of ATP, assists in protein folding. Co-expression of aldehyde dehydrogenase with GroEL/GroES promotes folding and higher production of protein in soluble form. GroES and GroEL bind to the protein substrate, enclosing it in a cage like fold. The target protein after folding gets dissociated from the cage and is accompanied with hydrolysis of adenosine triphosphate (ATP) to adenosine diphosphate (ADP). |
| Biochem/physiol Actions: | The antibody does not cross-react with GroES and bovine HSP 70. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | GroEL protein belongs to the folding chaperonin family. It is a 58.3 kDa protein with apical, intermediate and equatorial functional domains. |
| Immunogen: | purified recombinant GroEL produced in E. coli. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |